Chen Junfeng, Li Ke, Shon Ji Seon Lucy, Zimmerman Steven C
Department of Chemistry, University of Illinois, Urbana, Illinois 61801, United States.
Center for Biophysics and Quantitative Biology, University of Illinois, Urbana, Illinois 61801, United States.
J Am Chem Soc. 2020 Mar 11;142(10):4565-4569. doi: 10.1021/jacs.9b13997. Epub 2020 Feb 28.
Combining synthetic chemistry and biocatalysis is a promising but underexplored approach to intracellular catalysis. We report a strategy to codeliver a single-chain nanoparticle (SCNP) catalyst and an exogenous enzyme into cells for performing bioorthogonal reactions. The nanoparticle and enzyme reside in endosomes, creating engineered artificial organelles that manufacture organic compounds intracellularly. This system operates in both concurrent and tandem reaction modes to generate fluorophores or bioactive agents. The combination of SCNP and enzymatic catalysts provides a versatile tool for intracellular organic synthesis with applications in chemical biology.
将合成化学与生物催化相结合是一种用于细胞内催化的有前景但尚未充分探索的方法。我们报告了一种将单链纳米颗粒（SCNP）催化剂和外源酶共递送进细胞以进行生物正交反应的策略。纳米颗粒和酶存在于内体中，形成在细胞内制造有机化合物的工程化人工细胞器。该系统以并发和串联反应模式运行以生成荧光团或生物活性剂。SCNP与酶催化剂的结合为细胞内有机合成提供了一种通用工具，可应用于化学生物学。
