• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

猪口腔黏膜来源细胞的转录组学和形态学分析——体外模型研究

Transcriptomic and Morphological Analysis of Cells Derived from Porcine Buccal Mucosa-Studies on an In Vitro Model.

作者信息

Bryja Artur, Latosiński Grzegorz, Jankowski Maurycy, Angelova Volponi Ana, Mozdziak Paul, Shibli Jamil A, Bryl Rut, Spaczyńska Julia, Piotrowska-Kempisty Hanna, Krawiec Krzysztof, Kempisty Bartosz, Dyszkiewicz-Konwińska Marta

机构信息

Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland.

Institute of Computing Science, Poznan University of Technology, 60-965 Poznań, Poland.

出版信息

Animals (Basel). 2020 Dec 24;11(1):15. doi: 10.3390/ani11010015.

DOI:10.3390/ani11010015
PMID:33374146
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7824432/
Abstract

Transcriptional analysis and live-cell imaging are a powerful tool to investigate the dynamics of complex biological systems. In vitro expanded porcine oral mucosal cells, consisting of populations of epithelial and connective lineages, are interesting and complex systems for study via microarray transcriptomic assays to analyze gene expression profile. The transcriptomic analysis included 56 ontological groups with particular focus on 7 gene ontology groups that are related to the processes of differentiation and development. Most analyzed genes were upregulated after 7 days and downregulated after 15 and 30 days of in vitro culture. The performed transcriptomic analysis was then extended to include automated analysis of differential interference contrast microscopy (DIC) images obtained during in vitro culture. The analysis of DIC imaging allowed to identify the different populations of keratinocytes and fibroblasts during seven days of in vitro culture, and it was possible to evaluate the proportion of these two populations of cells. Porcine mucosa may be a suitable model for reference research on human tissues. In addition, it can provide a reference point for research on the use of cells, scaffolds, or tissues derived from transgenic animals for applications in human tissues reconstruction.

摘要

转录分析和活细胞成像技术是研究复杂生物系统动态变化的有力工具。体外扩增的猪口腔黏膜细胞由上皮和结缔组织谱系群体组成，是通过微阵列转录组分析来分析基因表达谱的有趣且复杂的研究系统。转录组分析包括56个本体论组，特别关注与分化和发育过程相关的7个基因本体论组。大多数分析的基因在体外培养7天后上调，在15天和30天后下调。然后将进行的转录组分析扩展到包括对体外培养期间获得的微分干涉对比显微镜（DIC）图像的自动分析。对DIC成像的分析能够在体外培养的7天内识别角质形成细胞和成纤维细胞的不同群体，并且可以评估这两种细胞群体的比例。猪黏膜可能是用于人体组织参考研究的合适模型。此外，它可以为研究使用来自转基因动物的细胞、支架或组织在人体组织重建中的应用提供参考点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/159fc36ed30b/animals-11-00015-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/8a085ed4f030/animals-11-00015-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/3cfa5dc96c71/animals-11-00015-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/4bd4fdf14a96/animals-11-00015-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/32adb80a5468/animals-11-00015-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/01a991740c6f/animals-11-00015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/db8074444a44/animals-11-00015-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/41e96cf2681c/animals-11-00015-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/9188a8c3a866/animals-11-00015-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/bf7f6b84856f/animals-11-00015-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/fa871eae383b/animals-11-00015-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/92b50d42f96b/animals-11-00015-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/448656699ad0/animals-11-00015-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/3e3234f297d3/animals-11-00015-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/b3212b5149c8/animals-11-00015-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/90467c0b864e/animals-11-00015-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/159fc36ed30b/animals-11-00015-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/8a085ed4f030/animals-11-00015-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/3cfa5dc96c71/animals-11-00015-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/4bd4fdf14a96/animals-11-00015-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/32adb80a5468/animals-11-00015-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/01a991740c6f/animals-11-00015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/db8074444a44/animals-11-00015-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/41e96cf2681c/animals-11-00015-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/9188a8c3a866/animals-11-00015-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/bf7f6b84856f/animals-11-00015-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/fa871eae383b/animals-11-00015-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/92b50d42f96b/animals-11-00015-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/448656699ad0/animals-11-00015-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/3e3234f297d3/animals-11-00015-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/b3212b5149c8/animals-11-00015-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/90467c0b864e/animals-11-00015-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/954b/7824432/159fc36ed30b/animals-11-00015-g016.jpg

相似文献

1
Transcriptomic and Morphological Analysis of Cells Derived from Porcine Buccal Mucosa-Studies on an In Vitro Model.猪口腔黏膜来源细胞的转录组学和形态学分析——体外模型研究
Animals (Basel). 2020 Dec 24;11(1):15. doi: 10.3390/ani11010015.
2
Genes involved in regulation of cellular metabolic processes, signaling and adhesion are the markers of porcine buccal pouch mucosal cells long-term primary cultured .参与细胞代谢过程、信号转导和黏附调节的基因是猪颊囊黏膜细胞长期原代培养的标志物。
J Biol Regul Homeost Agents. 2018 Sep-Oct;32(5):1129-1141.
3
New Gene Markers Involved in Molecular Processes of Tissue Repair, Response to Wounding and Regeneration Are Differently Expressed in Fibroblasts from Porcine Oral Mucosa during Long-Term Primary Culture.参与组织修复、伤口反应和再生分子过程的新基因标记物在猪口腔黏膜成纤维细胞长期原代培养过程中表达存在差异。
Animals (Basel). 2020 Oct 22;10(11):1938. doi: 10.3390/ani10111938.
4
New Gene Markers for Metabolic Processes and Homeostasis in Porcine Buccal Pouch Mucosa during Cells Long Term-Cultivation-A Primary Culture Approach.猪颊囊黏膜细胞长期培养中代谢过程和稳态的新基因标志物——一种原代培养方法。
Int J Mol Sci. 2018 Mar 29;19(4):1027. doi: 10.3390/ijms19041027.
5
Expression of cell mitotic progression proteins and keratinocyte markers in porcine buccal pouch mucosal cells during short-term, real-time primary culture.猪颊囊黏膜细胞短期实时原代培养过程中细胞有丝分裂进程蛋白和角质形成细胞标志物的表达
J Biol Regul Homeost Agents. 2017 Apr-Jun;31(2):297-309.
6
Gene expression regulating lipopolysaccharide and lipid metabolic processes in porcine oral mucosal cells in long-term primary in vitro culture.长期原代体外培养猪口腔黏膜细胞中基因表达调控脂多糖和脂代谢过程。
J Biol Regul Homeost Agents. 2019;33(3):695-706.
7
Differential expression and distribution of cytokeratins and vimentin in buccal pouch mucosal cells during real-time cell proliferation: research based on a porcine model.实时细胞增殖过程中颊囊黏膜细胞角蛋白和波形蛋白的差异表达与分布：基于猪模型的研究
J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4):951-960.
8
Expression of genes responsible for cell morphogenesis involved in differentiation in porcine buccal pouch mucosal cells during long-term primary culture and real-time proliferation in vitro.猪颊囊黏膜细胞长期原代培养及体外实时增殖过程中参与分化的细胞形态发生相关基因的表达
J Biol Regul Homeost Agents. 2017 Oct-Dec;31(4):855-864.
9
Effects of subepithelial fibroblasts on epithelial differentiation in human skin and oral mucosa: heterotypically recombined organotypic culture model.皮下成纤维细胞对人皮肤和口腔黏膜上皮分化的影响：异型重组器官型培养模型
Plast Reconstr Surg. 2003 Sep;112(3):784-92. doi: 10.1097/01.PRS.0000069710.48139.4E.
10
New Molecular Markers Involved in Regulation of Ovarian Granulosa Cell Morphogenesis, Development and Differentiation during Short-Term Primary In Vitro Culture-Transcriptomic and Histochemical Study Based on Ovaries and Individual Separated Follicles.短期体外原代培养过程中调控卵巢颗粒细胞形态发生、发育和分化的新分子标记物：基于卵巢和单个分离卵泡的转录组学和组织化学研究。
Int J Mol Sci. 2019 Aug 15;20(16):3966. doi: 10.3390/ijms20163966.

本文引用的文献

1
Biology of soft tissue repair: gingival epithelium in wound healing and attachment to the tooth and abutment surface.软组织修复生物学：愈合过程中的牙龈上皮与牙及基台表面的附着。
Eur Cell Mater. 2019 Aug 14;38:63-78. doi: 10.22203/eCM.v038a06.
2
Establishment of porcine and human expanded potential stem cells.猪和人诱导多能干细胞的建立。
Nat Cell Biol. 2019 Jun;21(6):687-699. doi: 10.1038/s41556-019-0333-2. Epub 2019 Jun 3.
3
Human saliva stimulates skin and oral wound healing in vitro.人唾液可促进体外皮肤和口腔创面愈合。
J Tissue Eng Regen Med. 2019 Jun;13(6):1079-1092. doi: 10.1002/term.2865. Epub 2019 May 2.
4
Global gene expression of histologically normal primary skin cells from BCNS subjects reveals "single-hit" effects that are influenced by rapamycin.基底细胞痣综合征（BCNS）患者组织学正常的原代皮肤细胞的全基因组表达揭示了受雷帕霉素影响的“单次打击”效应。
Oncotarget. 2019 Feb 15;10(14):1360-1387. doi: 10.18632/oncotarget.26640.
5
Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.活细胞成像技术在亚细胞结构定量评估多能干细胞中的应用。
Sci Rep. 2019 Feb 11;9(1):1777. doi: 10.1038/s41598-018-37779-x.
6
Sphere‑forming assay vs. organoid culture: Determining long‑term stemness and the chemoresistant capacity of primary colorectal cancer cells.球体形成分析与类器官培养：鉴定原发性结直肠癌细胞的长期干性和化学抗性能力。
Int J Oncol. 2019 Mar;54(3):893-904. doi: 10.3892/ijo.2019.4683. Epub 2019 Jan 11.
7
Use of a pre-vascularised oral mucosal cell sheet for promoting cutaneous burn wound healing.应用血管化口腔黏膜细胞片促进皮肤烧伤创面愈合。
Theranostics. 2018 Nov 10;8(20):5703-5712. doi: 10.7150/thno.28754. eCollection 2018.
8
Genes involved in regulation of cellular metabolic processes, signaling and adhesion are the markers of porcine buccal pouch mucosal cells long-term primary cultured .参与细胞代谢过程、信号转导和黏附调节的基因是猪颊囊黏膜细胞长期原代培养的标志物。
J Biol Regul Homeost Agents. 2018 Sep-Oct;32(5):1129-1141.
9
Cells with stemness features are generated from in vitro transformed human fibroblasts.具有干细胞特性的细胞是从体外转化的人成纤维细胞中产生的。
Sci Rep. 2018 Sep 14;8(1):13838. doi: 10.1038/s41598-018-32197-5.
10
Identification of rare sequence variation underlying heritable pulmonary arterial hypertension.遗传性肺动脉高压相关罕见序列变异的鉴定。
Nat Commun. 2018 Apr 12;9(1):1416. doi: 10.1038/s41467-018-03672-4.