Laboratory of Toxicology, Department of Pharmaceutical Sciences, Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, Brazil.
Unity for Characterization and Analysis, Institute for Research in Pharmaceuticals and Medications, Federal University of Paraíba, João Pessoa 58051-900, Brazil.
Toxins (Basel). 2020 Dec 23;13(1):4. doi: 10.3390/toxins13010004.
This study evaluated the efficacy of potentially probiotic fruit-derived isolates, namely, 108, 49, and 111, to remove aflatoxin M (AFM) from a phosphate buffer solution (PBS; spiked with 0.15 µg/mL AFM). The efficacy of examined isolates (approximately 10 cfu/mL) as viable and non-viable cells (heat-killed; 100 °C, 1 h) to remove AFM was measured after 1 and 24 h at 37 °C. The recovery of AFM bound to bacterial cells after washing with PBS was also evaluated. Levels of AFM in PBS were measured with high-performance liquid chromatography. Viable and non-viable cells of all examined isolates were capable of removing AFM in PBS with removal percentage values in the range of 73.9-80.0% and 72.9-78.7%, respectively. Viable and non-viable cells of all examined isolates had similar abilities to remove AFM. Only 108 showed higher values of AFM removal after 24 h for both viable and non-viable cells. Percentage values of recovered AFM from viable and non-viable cells after washing were in the range of 13.4-60.6% and 10.9-47.9%, respectively. 49 showed the highest AFM retention capacity after washing. 108, 49, and 111 could have potential application to reduce AFM to safe levels in foods and feeds. The cell viability of examined isolates was not a pre-requisite for their capacity to remove and retain AFM.
本研究评估了潜在益生菌水果分离株（即 108、49 和 111）从磷酸盐缓冲溶液（PBS；用 0.15µg/mL AFM 污染）中去除黄曲霉毒素 M（AFM）的功效。在 37°C 下孵育 1 和 24 小时后，测量了经检验的分离株（约 10cfu/mL）作为活菌和死菌（热杀死；100°C，1 小时）去除 AFM 的功效。还评估了用 PBS 洗涤后结合在细菌细胞上的 AFM 的回收情况。用高效液相色谱法测量 PBS 中的 AFM 水平。所有检验分离株的活菌和死菌都能够在 PBS 中去除 AFM，去除率分别为 73.9-80.0%和 72.9-78.7%。所有检验的分离株的活菌和死菌去除 AFM 的能力相似。只有 108 在活菌和死菌培养 24 小时后显示出更高的 AFM 去除值。洗涤后从活菌和死菌中回收的 AFM 的百分比值分别为 13.4-60.6%和 10.9-47.9%。49 在洗涤后显示出最高的 AFM 保留能力。108、49 和 111 可能有潜力应用于降低食品和饲料中 AFM 的安全水平。检验分离株的细胞活力不是其去除和保留 AFM 能力的前提条件。
