Azeem Nimra, Nawaz Muhammad, Anjum Aftab Ahmad, Saeed Shagufta, Sana Saba, Mustafa Amina, Yousuf Muhammad Rizwan
Department of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, Punjab, Pakistan.
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore 54000, Punjab, Pakistan.
Animals (Basel). 2019 Apr 15;9(4):166. doi: 10.3390/ani9040166.
Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates ( PDP 10, FYP 38, PDP 24, PL 53, PL 120, and PL 149) were tested for activity against toxigenic W-7.1 (AFB1 producer) by well diffusion assay. Only three isolates (PL 53, PL 120, and PL 149) had activity against W-7.1. The ameliorative effect of these probiotic isolates on AFB1 production was determined by co-culturing fungus with lactobacilli for 12 days, followed by aflatoxin quantification by high-performance liquid chromatography. In vitro AFB1 binding capacities of lactobacilli were determined by their incubation with a standard amount of AFB1 in phosphate buffer saline at 37 °C for 2 h. AFB1 binding capacities of isolates ranged from 28-65%. Four isolates (PDP 10, PDP 24, PL 120, and PL 149) also ceased aflatoxin production completely, whereas PL 53 showed 55% reduction in AFB1 production as compared to control. The present study demonstrated PL 149 to be an effective candidate AFB1 binding agent against . These findings further support the binding ability of lactic acid bacteria for dietary contaminants.
人类食品和动物饲料中的黄曲霉毒素污染对公共安全构成威胁。黄曲霉毒素B1（AFB1）对家禽生产尤其具有破坏性，进而影响巴基斯坦的经济发展。本研究评估了本土鉴定的益生菌乳酸菌对AFB1的体外结合能力。通过打孔扩散法测试了六种分离株（PDP 10、FYP 38、PDP 24、PL 53、PL 120和PL 149）对产毒W - 7.1（AFB1产生菌）的活性。只有三种分离株（PL 53、PL 120和PL 149）对W - 7.1具有活性。通过将真菌与乳酸菌共培养12天，然后用高效液相色谱法定量黄曲霉毒素，来确定这些益生菌分离株对AFB1产生的改善作用。通过在37℃下于磷酸盐缓冲盐水中将乳酸菌与标准量的AFB1孵育2小时，来测定乳酸菌的体外AFB1结合能力。分离株的AFB1结合能力范围为28 - 65%。四种分离株（PDP 10、PDP 24、PL 120和PL 149）也完全停止了黄曲霉毒素的产生，而与对照相比，PL 53的AFB1产量降低了55%。本研究表明PL 149是一种有效的抗AFB1结合剂。这些发现进一步支持了乳酸菌对膳食污染物的结合能力。
