Alexandrov Oleg S, Razumova Olga V, Karlov Gennady I
All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.
Plants (Basel). 2020 Dec 23;10(1):4. doi: 10.3390/plants10010004.
5S rDNA is organized as a cluster of tandemly repeated monomers that consist of the conservative 120 bp coding part and non-transcribed spacers (NTSs) with different lengths and sequences among different species. The polymorphism in the 5S rDNA NTSs of closely related species is interesting for phylogenetic and evolutional investigations, as well as for the development of molecular markers. In this study, the 5S rDNA NTSs were amplified with universal 5S1/5S2 primers in some species of the Elaeagnaceae Adans. family. The polymerase chain reaction (PCR) products of five species had similar lengths near 310 bp and were different from (L.) Nutt. and (Pusch.) Nutt. samples (260 bp and 215 bp, respectively). The PCR products were cloned and sequenced. An analysis of the sequences revealed that intraspecific levels of NTS identity are high (approximately 95-96%) and similar in the L. species. In , this level was slightly lower due to the differences in the poly-T region. Moreover, the intergeneric and intervarietal NTS identity levels were studied and compared. Significant differences between species (except Thunb. and Thunb.) and genera were found. Herein, a range of the NTS features is discussed. This study is another step in the investigation of the molecular evolution of Elaeagnaceae and may be useful for the development of species-specific DNA markers in this family.
5S核糖体DNA以串联重复单体簇的形式组织，这些单体由保守的120个碱基对的编码部分和非转录间隔区（NTSs）组成，不同物种间NTSs的长度和序列各异。近缘物种5S核糖体DNA非转录间隔区的多态性对于系统发育和进化研究以及分子标记的开发具有重要意义。在本研究中，利用通用引物5S1/5S2对胡颓子科的一些物种进行了5S核糖体DNA非转录间隔区的扩增。五个物种的聚合酶链反应（PCR）产物长度相似，接近310碱基对，与披针叶胡颓子（L.）Nutt.和沙棘（Pusch.）Nutt.的样本不同（分别为260碱基对和215碱基对）。对PCR产物进行了克隆和测序。序列分析表明，种内非转录间隔区的一致性水平较高（约95 - 96%），在披针叶胡颓子属物种中相似。在沙棘中，由于多聚T区域的差异，该水平略低。此外，还研究并比较了属间和种间非转录间隔区的一致性水平。发现物种（除海滨胡颓子Thunb.和薄叶胡颓子Thunb.外）和属之间存在显著差异。本文讨论了一系列非转录间隔区的特征。本研究是胡颓子科分子进化研究的又一步，可能有助于该科物种特异性DNA标记的开发。
