Alexandrov Oleg S, Karlov Gennady I
All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.
Plants (Basel). 2021 Dec 10;10(12):2713. doi: 10.3390/plants10122713.
The L. species are trees and bushes that mainly grow in temperate zones of Western Europe; Minor, Central, and Southeast Asia; the Far East; and North America. Some species are used as fruit or ornamental plants and have economic value. Problems with the identification of species in the genus by molecular genetical methods arise in the study of populations, systematics, breeding, and other areas of plant science and practice. Recently, the polymorphism of 5S ribosomal DNA non-transcribed spacers (5S rDNA NTSs) in Elaeagnaceae Adans. has been described. The results were used in our study as a basis for development of new species-specific molecular markers for some members of the genus. The author's method was applied for finding regions that were potentially applicable for species-specific primer design. As a result, some species-specific molecular markers were developed for L., Bernh., Thunb., and Thunb. These markers were tested in a range of samples and showed the presence of amplified fragments in lanes of the marked species only. Samples of other species showed no amplifications. Thus, the developed markers may be useful for the species identification of the studied plants in botanical, dendrological, and genetic research (especially in a leafless period of year), as well as in breeding and hybridization experiments.
胡颓子属植物为乔木和灌木，主要生长在西欧的温带地区；小亚细亚、中亚和东南亚地区；远东地区以及北美洲。一些物种被用作水果或观赏植物，具有经济价值。在植物科学和实践的种群研究、系统学、育种及其他领域中，利用分子遗传学方法鉴定该属物种时会出现问题。最近，已描述了胡颓子科5S核糖体DNA非转录间隔区（5S rDNA NTSs）的多态性。这些结果被用于我们的研究，作为为该属一些成员开发新的物种特异性分子标记的基础。应用作者的方法来寻找可能适用于物种特异性引物设计的区域。结果，为沙棘属、沙棘、沙棘、沙棘开发了一些物种特异性分子标记。这些标记在一系列样本中进行了测试，结果显示仅在标记物种的泳道中有扩增片段。其他物种的样本未显示扩增。因此，开发的标记可用于植物学、树木学和遗传学研究（尤其是在一年中的无叶期）中对所研究植物的物种鉴定，以及育种和杂交实验。
