Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Laboratory of Fruit Trees Disease, Institute of Economic Forestry, Xinjiang Academy of Forestry Sciences, Urumqi 830063, China.
Viruses. 2023 Jan 17;15(2):267. doi: 10.3390/v15020267.
Chinese jujube ( Mill.) is a widely grown fruit crop at Aksu in Xinjiang Uygur Autonomous Region of China. Viral disease-like symptoms are common on jujube plants. Here, for the first time, we report a virus tentatively named persimmon ampelovirus jujube isolate (PAmpV-Ju) infecting jujube plants. The virus was identified using high-throughput sequencing from a jujube plant (ID: AKS15) and molecularly related to viruses in the family . The genomic sequences of two PAmpV-Ju variants named AKS15-20 and AKS15-17 were determined by RT-PCR amplifications. The genome structure of PAmpV-Ju was identical to that of a recently reported persimmon ampelovirus (PAmpV) and consisted of seven open reading frames. The genomes of AKS15-20 and AKS15-17 shared 83.7% nt identity with each other, and the highest nt sequence identity of 79% with two variants of PAmpV. The incidence of PAmpV-Ju on Aksu jujube plants was evaluated by RT-PCR assays. The phylogenetic analysis of amplified partial sequences coding for polymerase, HSP70h, and CP revealed two phylogenetic clades represented by AKS15-20 and AKS15-17. Our study provides important evidence for understanding viruses infecting jujube plants and establishing efficient measures to prevent virus spread.
中国红枣（Mill.）是中国新疆维吾尔自治区阿克苏广泛种植的一种水果作物。枣树常见病毒病样症状。在这里，我们首次报道了一种暂定名为柿丛矮化病毒枣分离物（PAmpV-Ju）感染枣树。该病毒是通过高通量测序从一棵枣树（ID：AKS15）中鉴定出来的，与家族中的病毒分子上相关。通过 RT-PCR 扩增，确定了两个名为 AKS15-20 和 AKS15-17 的 PAmpV-Ju 变体的基因组序列。PAmpV-Ju 的基因组结构与最近报道的柿丛矮化病毒（PAmpV）相同，由七个开放阅读框组成。AKS15-20 和 AKS15-17 的基因组共享 83.7%的 nt 同一性，与两个 PAmpV 变体的最高 nt 序列同一性为 79%。通过 RT-PCR 检测评估了 PAmpV-Ju 在阿克苏枣树上的发病率。聚合酶、HSP70h 和 CP 编码的扩增部分序列的系统发育分析显示，以 AKS15-20 和 AKS15-17 为代表的两个系统发育枝。我们的研究为了解感染枣树的病毒提供了重要证据，并为制定有效的病毒传播防治措施提供了依据。
