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基于16S rRNA测序技术研究黄连解毒汤对SD大鼠肠道微生物群落组成的影响

Study on the Effect of Huanglian Jiedu Decoction on the Composition of Gut Microflora in SD Rats Based on 16S rRNA Sequencing.

作者信息

Du Haiyang, Zhang Gengxin, Yang Guangyong, Tian Weiyi, Wang Wenjia, Wang Ping, He Guangzhi

机构信息

College of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.

出版信息

Evid Based Complement Alternat Med. 2020 Dec 8;2020:8872439. doi: 10.1155/2020/8872439. eCollection 2020.

DOI:10.1155/2020/8872439
PMID:33376503
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7738789/
Abstract

OBJECTIVE

To study the effects of Huanglian Jiedu Decoction (HJD) on gut microflora of SD rats.

METHOD

After 3 days of adaptive feeding, 36 rats were randomly divided into 4 groups, namely, the normal control group (NC, 10 mL/kg, distilled water), high_HJD dose group (H_HJD, 6.25 g/kg, weight ratio between crude drug and rat), medium_HJD dose group (M_HJD, 3.125 g/kg), and low_HJD dose group (L_HJD, 1.56 g/kg), and each group consisted of 9 mice. The HJD groups were then treated with orally administered HJD for 21 days, while the NC group was treated with distilled water. After 7 days, 14 days, and 21 days of the experiment and after 12 hours of fasting and water deprivation, 3 SD rats in each group were randomly sacrificed by cervical dislocation and sterile operation to collect stool faces. Sample DNA was extracted by Fecal total DNA extraction kit, sequenced using Illumina MiSeq platform, and analyzed.

RESULTS

The abundance of , , , and decreased, while that of , , , , , , , , and increased after using H_HJD. The abundance of , , and decreased, while that of , , Escherichia_Shigella, , , , , and increased after using M_HJD. The abundance of , , , and decreased, while that of Escherichia_Shigella, , , , , , , , , , and increased after using L_HJD.

CONCLUSION

HJD can regulate gut microbiota, and its effect varies with different dosage and duration of medication.

摘要

目的

研究黄连解毒汤对SD大鼠肠道菌群的影响。

方法

适应性喂养3天后，将36只大鼠随机分为4组，即正常对照组（NC，10 mL/kg，蒸馏水）、黄连解毒汤高剂量组（H_HJD，6.25 g/kg，生药与大鼠体重比）、黄连解毒汤中剂量组（M_HJD，3.125 g/kg）和黄连解毒汤低剂量组（L_HJD，1.56 g/kg），每组9只。黄连解毒汤组灌胃给予黄连解毒汤21天，NC组给予蒸馏水。实验7天、14天、21天后，禁食禁水12小时，每组随机处死3只SD大鼠，无菌操作收集粪便。采用粪便总DNA提取试剂盒提取样本DNA，使用Illumina MiSeq平台进行测序并分析。

结果

使用H_HJD后，[具体菌名1]、[具体菌名2]、[具体菌名3]、[具体菌名4]丰度降低，[具体菌名5]、[具体菌名6]、[具体菌名7]、[具体菌名8]、[具体菌名9]、[具体菌名10]、[具体菌名11]、[具体菌名12]、[具体菌名13]丰度增加。使用M_HJD后，[具体菌名14]、[具体菌名15]、[具体菌名16]丰度降低，[具体菌名17]、[具体菌名18]、大肠埃希菌志贺菌属、[具体菌名19]、[具体菌名20]、[具体菌名21]、[具体菌名22]、[具体菌名23]丰度增加。使用L_HJD后，[具体菌名24]、[具体菌名25]、[具体菌名26]、[具体菌名27]丰度降低，大肠埃希菌志贺菌属、[具体菌名28]、[具体菌名29]、[具体菌名30]、[具体菌名31]、[具体菌名32]、[具体菌名33]、[具体菌名34]、[具体菌名35]、[具体菌名36]、[具体菌名37]丰度增加。

结论

黄连解毒汤可调节肠道菌群，其作用随用药剂量和疗程不同而有所差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03bf/7738789/01068381d8ea/ECAM2020-8872439.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03bf/7738789/c9ccc63e3d26/ECAM2020-8872439.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03bf/7738789/01068381d8ea/ECAM2020-8872439.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03bf/7738789/c9ccc63e3d26/ECAM2020-8872439.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/03bf/7738789/01068381d8ea/ECAM2020-8872439.002.jpg

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