Czerwiński Michał, Bednarska-Czerwińska Anna, Zmarzły Nikola, Boroń Dariusz, Oplawski Marcin, Grabarek Beniamin Oskar
American Medical Clinic, 40-600 Katowice, Poland.
Gyncentrum Fertility Clinic, 40-121 Katowice, Poland.
J Clin Med. 2021 Oct 26;10(21):4939. doi: 10.3390/jcm10214939.
Disruption of the dopaminergic system leads to many diseases, including cancer. Dopamine and its receptors are involved in the regulation of proliferation, cell death, invasion, and migration. Better understanding of the mechanisms involved in these processes could reveal new molecular markers and therapeutic targets. The aim of this study was to determine the expression profile of dopamine-related genes and proteins in endometrial cancer and to assess whether miRNAs are involved in its regulation. Sixty women were recruited for the study: 30 with endometrial cancer and 30 without cancer. The expression profiles of dopamine-related genes were determined in endometrial tissue samples using microarrays and qRT-PCR. Then, protein concentration was determined with the ELISA test. In the last step, miRNA detection was performed using microarrays. The matching of miRNAs to the studied genes was carried out using the TargetScan tool. The analysis showed DRD2 and DRD3 overexpression, with a reduction in DRD5 expression, which could be due to miR-15a-5p, miR-141-3p, miR-4640-5p, and miR-221-5p activity. High levels of OPRK1 and CXCL12, related to the activity of miR-124-3p.1 and miR-135b-5p, have also been reported. Low COMT expression was probably not associated with miRNA regulation in endometrial cancer.
多巴胺能系统的破坏会导致包括癌症在内的多种疾病。多巴胺及其受体参与细胞增殖、细胞死亡、侵袭和迁移的调节。更好地理解这些过程中涉及的机制可能会揭示新的分子标志物和治疗靶点。本研究的目的是确定多巴胺相关基因和蛋白质在子宫内膜癌中的表达谱，并评估miRNA是否参与其调节。本研究招募了60名女性：30名患有子宫内膜癌，30名未患癌症。使用微阵列和qRT-PCR测定子宫内膜组织样本中多巴胺相关基因的表达谱。然后，用ELISA试验测定蛋白质浓度。在最后一步，使用微阵列进行miRNA检测。使用TargetScan工具进行miRNA与研究基因的匹配。分析显示DRD2和DRD3过表达，而DRD5表达降低，这可能归因于miR-15a-5p、miR-141-3p、miR-4640-5p和miR-221-5p的活性。还报道了与miR-124-3p.1和miR-135b-5p活性相关的高水平OPRK1和CXCL12。子宫内膜癌中低水平的COMT表达可能与miRNA调节无关。
