Dubie Teshager, Amare Tsedale
Research and Community Service, Samara University, P.O. Box 132, Samara, Ethiopia.
College of Veterinary Medicine, Samara University, P.O. Box 132, Samara, Ethiopia.
Vet Med Int. 2020 Dec 9;2020:8847728. doi: 10.1155/2020/8847728. eCollection 2020.
On the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for the reason of the presence of FMD outbreak case report during the study period. Totally, 27 FMD clinical samples were collected from affected study population during field outbreak. Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. Finally, the PCR products were visualized with UV illumination and imaged with gel documentation system.
The current study results revealed that out of 18 clinical samples subjected to virus isolation, 72.2% ( = 13) of these cultures exhibited FMDV-induced cytopathic effect (CPE) and the identified serotype was SAT-2 FMD virus. Out of 27 clinical samples tested by conventional RT-PCR, only 12 FMDV samples were found to be FMDV positive by universal primers. Out of 27 samples detected by conventional RT-PCR, only 12 FMDV samples were found to be FMDV positive by universal primers.
Our study finding indicated that FMDV is prevalent in the study area and FMDV serotype SAT-2 was the causality for the outbreaks of the disease in the study area. Hence, region-wise FMD outbreak investigation, further phylogenetic analysis, and vaccine matching field isolates should be carried out for effective vaccine development to control the disease.
基于口蹄疫病毒（FMDV）疫情病例，于2019年1月至2020年3月开展了一项横断面研究，旨在从研究区域采集样本，用于FMDV的分离、血清型鉴定和分子检测。由于研究期间有口蹄疫疫情报告，采用了目的抽样方法选择研究区域。在现场疫情期间，共从受影响的研究人群中采集了27份FMD临床样本。在这27份样本中，18份接种于培养的幼仓鼠肾（BHK - 21）单层细胞，所有27份样本均使用常规RT - PCR和一组特异性通用引物进行检测。最后，通过紫外线照射使PCR产物可视化，并用凝胶成像系统成像。
当前研究结果显示，在18份进行病毒分离的临床样本中，72.2%（即13份）的培养物表现出FMDV诱导的细胞病变效应（CPE），鉴定出的血清型为SAT - 2型FMD病毒。在27份通过常规RT - PCR检测的临床样本中，仅12份FMDV样本通过通用引物检测为FMDV阳性。
我们的研究结果表明，FMDV在研究区域流行，且FMDV血清型SAT - 2是该研究区域疾病爆发的病因。因此，应进行区域对口蹄疫疫情调查、进一步的系统发育分析以及使疫苗与现场分离株匹配，以开发有效的疫苗来控制该疾病。
