Yang Jiaxue, Liao Mengyuan, Hong Gaoying, Dai Shiqi, Shen Jiadi, Xie Haifeng, Chen Chen
Jiangsu Key Laboratory of Oral Diseases, Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Jiangsu Key Laboratory of Oral Diseases, Department of Endodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China.
ACS Omega. 2020 Dec 9;5(50):32540-32550. doi: 10.1021/acsomega.0c04762. eCollection 2020 Dec 22.
To investigate the effects of 3-aminopropyltriethoxysilane (APTES)- or (3-mercaptopropyl)trimethoxysilane (MPTS)-conditioned nanozirconia fillers on the mechanical properties of Bis-GMA-based resin composites. The conditioned fillers were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and thermodynamic calculations. They were then used to prepare Bis-GMA-based resin composites, whose flexural strength and elastic modulus were evaluated. The Cell Counting Kit-8 (CCK-8) assessed the composites' cytotoxicity. The FTIR spectra of the conditioned fillers showed new absorption bands at 1569 and 1100 cm, indicating successful grafting of APTES or MPTS onto nanozirconia. XPS confirmed the Zr-O-Si bonds in the APTES- or MPTS-conditioned fillers at contents of 2.02 and 6.98%, respectively. Thermodynamic calculations reaffirmed the chemical binding between the two silanes and nanozirconia fillers. Composites containing the conditioned nanozirconia fillers had significantly greater flexural strengths (APTES, 121.02 ± 8.31 MPa; MPTS, 132.80 ± 15.80 MPa; control, 94.84 ± 9.28 MPa) and elastic moduli (8.76 ± 0.52, 9.24 ± 0.60, and 7.44 ± 0.83 GPa, respectively) than a control with untreated fillers. The cytotoxicity assay identified no significant cytotoxicity by composites containing the conditioned fillers. Silanes were previously considered to be unable to chemically condition zirconia to bond with resin. Inclusion of APTES- or MPTS-conditioned nanozirconia fillers can improve the mechanical properties of Bis-GMA-based resin composites without obvious cytotoxicity in this study.
为研究3-氨丙基三乙氧基硅烷（APTES）或3-巯基丙基三甲氧基硅烷（MPTS）处理的纳米氧化锆填料对双酚A-双甲基丙烯酸缩水甘油酯（Bis-GMA）基树脂复合材料力学性能的影响。通过傅里叶变换红外光谱（FTIR）、X射线光电子能谱（XPS）和热力学计算对处理后的填料进行表征。然后用它们制备Bis-GMA基树脂复合材料，并评估其弯曲强度和弹性模量。细胞计数试剂盒-8（CCK-8）评估复合材料的细胞毒性。处理后填料的FTIR光谱在1569和1100 cm处出现新的吸收带，表明APTES或MPTS成功接枝到纳米氧化锆上。XPS证实APTES或MPTS处理的填料中Zr-O-Si键的含量分别为2.02%和6.98%。热力学计算再次证实了两种硅烷与纳米氧化锆填料之间的化学结合。含有处理后纳米氧化锆填料的复合材料的弯曲强度（APTES为121.02±8.31 MPa；MPTS为132.80±15.80 MPa；对照组为94.84±9.28 MPa）和弹性模量（分别为8.76±0.52、9.24±0.60和7.44±0.83 GPa）明显高于未处理填料的对照组。细胞毒性试验表明，含有处理后填料的复合材料无明显细胞毒性。硅烷以前被认为不能对氧化锆进行化学处理以使其与树脂结合。在本研究中，加入APTES或MPTS处理的纳米氧化锆填料可改善Bis-GMA基树脂复合材料的力学性能，且无明显细胞毒性。
