金表面上用于生物物理应用的细胞微图案化。

Micropatterning of Cells on Gold Surfaces for Biophysical Applications.

机构信息

University Ulm, Institute of Experimental Physics, Ulm, Baden-Württemberg 89081, Germany.

出版信息

STAR Protoc. 2020 Sep 17;1(3):100106. doi: 10.1016/j.xpro.2020.100106. eCollection 2020 Dec 18.

DOI:10.1016/j.xpro.2020.100106

PMID:33377002

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7756938/

Abstract

We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications.

摘要

我们开发了一种可重复的微图案化方法，用于在金上操纵和规范细胞形状和细胞间分离。我们使用甲氧基聚乙二醇硫醇（PEG-SH）创建了一个自组装单层，通过具有深紫外光的光掩模，可以将其氧化成所需的形状。氧化的 PEG 可以涂覆细胞外基质蛋白，并接种采用预定义形状的细胞。开发和分析的表面可用于广泛的生物物理应用。