无黏附水凝胶的单细胞微图案化。
Single-Cell Micropatterning by Non-fouling Hydrogels.
机构信息
Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China.
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, China.
出版信息
Methods Mol Biol. 2023;2689:65-70. doi: 10.1007/978-1-0716-3323-6_6.
Micropatterned substrate is a unique method for studying cell biology at the single-cell level. Using photolithography to create binary patterns of cell-adherent peptide surrounding by non-fouling cell-repellent poly(ethylene glycol) (PEG) hydrogel, this patterning method allows for controlling cell attachment with desired sizes and shapes up to 19 days. Here we provide the detailed procedure of fabrication for such patterns. This method will allow monitoring of prolonged reaction of single cells such as cell differentiation upon induction or time-resolved apoptosis stimulated by drug molecules for cancer treatment.
微图案化基底是一种在单细胞水平上研究细胞生物学的独特方法。通过光刻技术在非黏附细胞的聚乙二醇（PEG）水凝胶周围创建黏附肽的二元图案，这种图案化方法可控制细胞附着的大小和形状，最长可达 19 天。在此，我们提供了这种图案的详细制作过程。该方法将允许监测单个细胞的长期反应，例如诱导细胞分化或药物分子刺激的时间分辨细胞凋亡等。
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