研究发育期大鼠神经元饥饿诱导自噬的方案。
Protocol to study starvation-induced autophagy in developing rat neurons.
机构信息
Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, 75014 Paris, France.
GHU PARIS psychiatrie & neurosciences, 75014 Paris, France.
出版信息
STAR Protoc. 2021 Aug 7;2(3):100713. doi: 10.1016/j.xpro.2021.100713. eCollection 2021 Sep 17.
Autophagy is being involved in an increasing number of cellular pathways. It now appears that autophagy stimulation and inhibition have complex effects in neurons. Here, we present a simple yet powerful protocol to induce autophagy in primary neurons in culture by partial nutrient deprivation, in neurons with or without transfection of plasmids encoding the Longin domain of VAMP7 or a nanobody directed against VAMP7. Although limited to cells in culture, this protocol can facilitate the study of autophagy in neurons. For complete details on the use and execution of this protocol, please refer to Wojnacki et al. (2020).
自噬参与了越来越多的细胞通路。现在看来，自噬的刺激和抑制对神经元有复杂的影响。在这里，我们提出了一种简单而强大的方法，通过部分营养剥夺在培养的原代神经元中诱导自噬，无论是在转染编码 VAMP7 的 Longin 结构域的质粒还是针对 VAMP7 的纳米抗体的神经元中都是如此。虽然该方法仅限于培养的细胞，但它可以促进神经元中自噬的研究。有关此方案的使用和执行的完整详细信息，请参阅 Wojnacki 等人。(2020)。
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