Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
RNA Sequencing Core, Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
STAR Protoc. 2020 Oct 6;1(3):100123. doi: 10.1016/j.xpro.2020.100123. eCollection 2020 Dec 18.
Dual RNA-sequencing is a powerful technique to assess both bacterial and host transcriptomes in an unbiased way. We developed a protocol to perform Dual RNA-seq on -derived macrophage populations infected with . Here, we provide a practical step-by-step guide to execute the protocol on Mtb-infected cells from a murine infection model. Our protocol can also be easily applied to perform Dual RNA-seq on -derived cells as well as different Mtb-infected host cell types. For complete details on the use and execution of this protocol, please refer to Pisu et al. (2020).
双 RNA 测序是一种强大的技术，可以不偏不倚地评估细菌和宿主的转录组。我们开发了一种在感染 的衍生巨噬细胞群体上进行双 RNA 测序的方案。在这里，我们提供了一个从鼠感染模型中执行该方案的实用分步指南。我们的方案也可以很容易地应用于衍生细胞以及不同的 Mtb 感染宿主细胞类型上进行双 RNA 测序。有关该方案的使用和执行的完整详细信息，请参阅 Pisu 等人（2020 年）。
