用于光遗传学控制细胞信号动力学的工程照明设备制作方案。

Protocol to Fabricate Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics.

机构信息

Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.

Graduate Program in Bioengineering, University of California, San Francisco and University of California, Berkeley, Berkeley, CA 94720, USA.

出版信息

STAR Protoc. 2020 Oct 21;1(3):100141. doi: 10.1016/j.xpro.2020.100141. eCollection 2020 Dec 18.

DOI:10.1016/j.xpro.2020.100141

PMID:33377035

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7757298/

Abstract

Optogenetic modulation of protein interactions enables spatiotemporal control of cellular signaling dynamics in a variety of biological systems. However, light patterning by standard microscopes is limited by their complexity, sample throughput, and cost. To address the need for low-cost, user-friendly, and high-throughput photopatterning, we have engineered devices for light activation at variable amplitudes (LAVA). This protocol describes the assembly of LAVA devices, which enable spatial and temporal control of optogenetic stimulation and cellular signaling dynamics in multiwell cell culture plates. For complete details on the use and execution of this protocol, please refer to Repina et al. (2020).

摘要

光遗传学调节蛋白质相互作用使各种生物系统中的细胞信号动力学的时空控制成为可能。然而，标准显微镜的光模式限制了它们的复杂性、样本通量和成本。为了解决低成本、用户友好和高通量光图案化的需求，我们设计了用于可变幅度光激活的设备（LAVA）。本协议描述了 LAVA 设备的组装，该设备可以在多孔细胞培养板中对光遗传学刺激和细胞信号动力学进行时空控制。有关此协议的使用和执行的完整详细信息，请参阅 Repina 等人。（2020）。