小神经元贴壁测序方案。

Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience (IoPPN), King's College London, London, UK.

STAR Protoc. 2020 Oct 22;1(3):100146. doi: 10.1016/j.xpro.2020.100146. eCollection 2020 Dec 18.

Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type.

从同一个神经元中获取电生理记录和基因表达信息（Patch-seq）为研究功能特性的转录相关性提供了独特的机会，反之亦然。在这里，我们提供了一个详细的 Patch-seq 方案，专门针对研究小中间神经元的特殊需求进行了定制。本方案重点介绍了在从膜片钳记录到细胞提取的转换过程中技术要求较高的步骤，描述并解决了成功收集小中间神经元的步骤，从而可以对这种关键细胞类型进行多模式的 Patch-seq 检测。