Affinity Purification of Label-free Tubulins from Egg Extracts.

Cytoplasmic extracts from unfertilized eggs have made important contributions to our understanding of microtubule dynamics, spindle assembly, and scaling. Until recently, these studies relied on the use of heterologous tubulin. This protocol allows for the purification of physiologically relevant tubulins in milligram yield, which are a complex mixture of isoforms with various post-translational modifications. The protocol is applicable to any cell or tissue of interest. For complete details on the use and execution of this protocol, please refer to Hirst et al. (2020).