Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
STAR Protoc. 2020 Nov 6;1(3):100159. doi: 10.1016/j.xpro.2020.100159. eCollection 2020 Dec 18.
Myeloablative gamma irradiation has traditionally been used to condition mice prior to bone marrow transplantation. However, irradiation induces high levels of inflammation that may alter patterns of reconstitution. In addition, gamma irradiators are being removed from many facilities for security reasons. Alternative conditioning regimens are thus needed. Here, we describe a protocol for the use of busulfan to condition mice for bone marrow transplantation and several of the variables to consider for effective implementation. For complete details on the use and execution of this protocol, please refer to Montecino-Rodriguez et al. (2019).
传统上，在进行骨髓移植之前，用清髓性γ射线照射来对小鼠进行预处理。然而，照射会引起高水平的炎症，从而可能改变重建的模式。此外，出于安全原因，许多设施正在拆除γ射线照射器。因此，需要替代的预处理方案。在这里，我们描述了一种使用白消安对小鼠进行骨髓移植预处理的方案，并介绍了一些在有效实施时需要考虑的变量。如需详细了解本方案的使用和实施，请参考 Montecino-Rodriguez 等人（2019 年）的文献。
