The New York Stem Cell Foundation Research Institute, 619 west 54 street, New York, NY 10019, USA.
STAR Protoc. 2020 Nov 10;1(3):100172. doi: 10.1016/j.xpro.2020.100172. eCollection 2020 Dec 18.
Given the critical roles of astrocytes in neuroinflammation and neurological diseases, models for studying human astrocyte biology are in increasing demand. Here, we present a protocol to isolate human astrocytes from induced pluripotent stem cell (iPSC)-based cultures, neural organoids, and primary tissue, using the surface marker CD49f. Moreover, we provide protocols for co-cultures of human iPSC-derived neurons and astrocytes, as well as for neurotoxicity assays that expose neurons to conditioned media from reactive astrocytes. For complete details on the use and execution of this protocol, please refer to Barbar et al. (2020).
鉴于星形胶质细胞在神经炎症和神经疾病中的关键作用，研究人类星形胶质细胞生物学的模型需求日益增加。在这里，我们提出了一种使用表面标记物 CD49f 从诱导多能干细胞 (iPSC) 培养物、神经类器官和原代组织中分离人星形胶质细胞的方案。此外，我们还提供了人 iPSC 衍生神经元和星形胶质细胞共培养以及神经毒性测定的方案，该测定将神经元暴露于反应性星形胶质细胞的条件培养基中。有关该方案使用和执行的完整详细信息，请参阅 Barbar 等人。（2020 年）。
