Department of Pathology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Department of Psychiatry, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
STAR Protoc. 2020 Dec 1;1(3):100190. doi: 10.1016/j.xpro.2020.100190. eCollection 2020 Dec 18.
This protocol establishes a tri-culture of hiPSC-derived neurons, astrocytes, and microglia for the study of cellular interactions during homeostasis, injury, and disease. This system allows for mechanistic studies that can identify the roles of individual cell types in disease and injury response in a physiologically relevant, all-human system. This protocol utilizes and modifies prior differentiations. Limitations include the prolonged maturation of human astrocytes and neurons and scalability. For complete details on the use and execution of this protocol, please refer to Ryan et al. (2020).
本方案建立了人诱导多能干细胞来源的神经元、星形胶质细胞和小胶质细胞的三细胞共培养体系，用于研究在稳态、损伤和疾病过程中的细胞相互作用。该系统允许进行机制研究，可在生理相关的全人体系中鉴定单个细胞类型在疾病和损伤反应中的作用。本方案利用并修改了之前的分化方法。其局限性包括人类星形胶质细胞和神经元的成熟时间延长以及可扩展性。有关使用和执行本方案的完整详细信息，请参阅 Ryan 等人（2020 年）。
