诱导创伤和测量表皮膜修复的方案。

Protocol to Induce Wounding and Measure Membrane Repair in Epidermis.

机构信息

Center for Stem Cell and Regenerative Medicine and Department of Cardiology of The Second Affiliated Hospital, Zhejiang University School of Medicine, 310058 Hangzhou, China.

School of Basic Medical Sciences, Zhejiang University School of Medicine, 310058 Hangzhou, China.

出版信息

STAR Protoc. 2020 Nov 21;1(3):100175. doi: 10.1016/j.xpro.2020.100175. eCollection 2020 Dec 18.

DOI:10.1016/j.xpro.2020.100175

PMID:33377069

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7757402/

Abstract

Efficient membrane repair after injury is essential for cell and animal survival. epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair . This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis. For details on the use and execution of this protocol, please refer to Meng et al. (2020).

摘要

高效的膜修复对于细胞和动物的生存至关重要。表皮细胞 hpy7 已成为研究膜修复分子机制的强大遗传系统。本方案描述了在表皮上进行划痕实验以及通过台盼蓝染色、共聚焦成像和数据分析来检测膜修复的详细方法。有关该方案使用和执行的详细信息，请参见 Meng 等人（2020 年）。