School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
STAR Protoc. 2022 Apr 19;3(2):101336. doi: 10.1016/j.xpro.2022.101336. eCollection 2022 Jun 17.
Live imaging is an important tool to track dynamic processes such as neuronal patterning events. Here, we describe a protocol for time-lapse microscopy analysis using neuronal migration and dendritic growth as examples. This protocol can provide detailed information for understanding cellular dynamics during postembryonic development in . For complete details on the use and execution of this protocol, please refer to Feng et al. (2020), Li et al. (2021), and Wang et al. (2021).
实时成像技术是追踪神经元模式形成等动态过程的重要工具。本文以神经元迁移和树突生长为例，描述了使用延时显微镜分析的方案。该方案可以为理解胚胎后发育过程中的细胞动态提供详细信息。如需了解本方案的使用和执行的完整详细信息，请参考 Feng 等人（2020 年）、Li 等人（2021 年）和 Wang 等人（2021 年）的研究。
