Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
STAR Protoc. 2020 Nov 25;1(3):100181. doi: 10.1016/j.xpro.2020.100181. eCollection 2020 Dec 18.
The purpose of this protocol is to describe the generation of missense mutations in mice using CRISPR-Cas9 technology. The current protocol focuses on the generation of a missense mutation, but it can be adapted to introduce any missense or nonsense mutation. For complete details on the use and execution of this protocol, please refer to Tummers et al. (2020).
本方案旨在描述使用 CRISPR-Cas9 技术在小鼠中产生错义突变的方法。本方案目前专注于产生一个错义突变，但也可以进行修改以引入任何错义或无义突变。如需使用和执行本方案的完整详细信息，请参考 Tummers 等人（2020 年）。
