新型基因工程动物模型的生成方法。
Novel methods for the generation of genetically engineered animal models.
机构信息
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA.
Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
出版信息
Bone. 2023 Feb;167:116612. doi: 10.1016/j.bone.2022.116612. Epub 2022 Nov 13.
Genetically modified mouse models have shaped our understanding of biological systems in both physiological and pathological conditions. For decades, mouse genome engineering has relied on transgenesis and spontaneous gene replacement in embryonic stem (ES) cells. While these technologies provided a wealth of knowledge, they remain imprecise and expensive to use. Recent advances in genome editing technologies such as the development of targetable nucleases, the improvement of delivery systems, and the simplification of targeting strategies now allow for the rapid, precise manipulation of the mouse genome. In this review article, we discuss novel methods and targeting strategies for the generation of mouse models for the study of bone and skeletal muscle biology.
基因修饰小鼠模型在生理和病理条件下改变了我们对生物系统的理解。几十年来，小鼠基因组工程一直依赖于转基因和胚胎干细胞（ES 细胞）中的自发基因替换。虽然这些技术提供了丰富的知识，但它们仍然不够精确，使用成本也很高。近年来，基因组编辑技术的进步，如靶向核酸酶的开发、递送系统的改进以及靶向策略的简化，使得快速、精确地操纵小鼠基因组成为可能。在这篇综述文章中，我们讨论了用于研究骨骼和骨骼肌生物学的小鼠模型的生成的新方法和靶向策略。
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