基于定量成像的琼脂平板上酵母生长和存活分析方案
A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates.
机构信息
Department of Biology, The University of Western Ontario, London, ON N6A 3K7, Canada.
Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada.
出版信息
STAR Protoc. 2020 Nov 25;1(3):100182. doi: 10.1016/j.xpro.2020.100182. eCollection 2020 Dec 18.
We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike. For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).
我们呈现了一个详细的方案，描述了通过定量分析点样实验来评估酵母细胞的生长和存活情况。这种简单的方法通过测量单个点样实验图像中定义大小的单个点内的细胞密度，可重复性地检测和量化生长的细微差异。我们的方案专门针对低通量应用进行了定制，可以轻松适应特定的实验条件，并且对酵母专家和非专家都适用。有关执行此方案的示例，请参见 DiGregorio 等人的研究（Di Gregorio 等人，2020）。
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