采用LiAc/SS载体DNA/PEG法进行微量滴定板转化。
Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.
作者信息
Gietz R Daniel, Schiestl Robert H
机构信息
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
出版信息
Nat Protoc. 2007;2(1):5-8. doi: 10.1038/nprot.2007.16.
PMID:17401331
Abstract
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.
摘要
在此，我们描述了一种适用于在96孔微量滴定板中转化酵母细胞的方法。该方法可针对多种应用进行调整，适用于高通量应用。一旦酵母细胞生长起来，根据所使用的热激条件，该过程可在2至3小时内完成。
Zotero 插件