Department of Biology, Maynooth University, National University of Ireland, Maynooth W23F2H6, Co. Kildare, Ireland.
Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth W23F2H6, Co. Kildare, Ireland.
STAR Protoc. 2020 Dec 3;1(3):100196. doi: 10.1016/j.xpro.2020.100196. eCollection 2020 Dec 18.
This protocol describes the comparative proteomic profiling of the spleen of wild type versus mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass spectrometric analysis and bioinformatic evaluation. For complete details on the use and execution of this protocol, please refer to Dowling et al. (2020).
本方案描述了野生型与肌营养不良症模型鼠的脾脏的比较蛋白质组学分析。我们详细说明了用于下向蛋白质组学质谱实验的样品制备过程，包括组织匀浆、蛋白质浓度测量、蛋白质消化以及干扰化学物质的去除。然后，我们描述了质谱分析和生物信息学评估的步骤。有关此方案的使用和执行的完整详细信息，请参阅 Dowling 等人（2020 年）。
