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基于 LC-MS/MS 分析的 COVID-19 患者呼吸道标本（VTM）中全球蛋白质组、病毒组和宏蛋白质组分析的方案。

Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis.

机构信息

Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi 110070, India.

Department of Virology, Institute of Liver and Biliary Sciences, New Delhi, India.

出版信息

STAR Protoc. 2022 Mar 18;3(1):101045. doi: 10.1016/j.xpro.2021.101045. Epub 2021 Nov 27.

DOI:10.1016/j.xpro.2021.101045
PMID:34870243
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8626227/
Abstract

In this protocol, we describe global proteome profiling for the respiratory specimen of COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. In this protocol, details for identifying host, viral, or bacterial proteome (Meta-proteome) are provided. Major steps of the protocol include virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry (HRMS)-based analysis, and downstream bioinformatics analysis. For complete details on the use and execution of this profile, please refer to Maras et al. (2021).

摘要

在本方案中，我们描述了用于 COVID-19 患者、疑似 COVID-19 患者和 H1N1 患者的呼吸道标本的全蛋白质组分析。本方案提供了鉴定宿主、病毒或细菌蛋白质组（宏蛋白质组）的详细信息。方案的主要步骤包括病毒失活、蛋白质定量和消化、肽脱盐、基于高分辨率质谱（HRMS）的分析以及下游生物信息学分析。有关该分析方案的使用和执行的完整详细信息，请参考 Maras 等人（2021 年）。

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