German Federal Institute for Risk Assessment, German Centre for the Protection of Laboratory Animals (Bf3R), Berlin 10589, Germany.
Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin 10117, Germany.
STAR Protoc. 2020 Dec 16;1(3):100224. doi: 10.1016/j.xpro.2020.100224. eCollection 2020 Dec 18.
Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in . Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated framework using open-source software. The assay utilizes whole 96-well fluorescence images taken with a high-content screening system. Alternatively, conventional fluorescence images can also be utilized with only a few adjustments. For complete details on the use and execution of this protocol, please refer to Wittkowski et al. (2019).
生长和后代数量是用于评估化学物质或基因引起的发育和生殖毒性的两种常见毒理学终点。在这里，我们提出了一种 96 小时高通量测定的方案，该方案使用开源软件在自动化框架内定量评估这两个终点。该测定利用高内涵筛选系统拍摄的全 96 孔荧光图像。或者，也可以仅进行少量调整后使用传统的荧光图像。如需详细了解该方案的使用和执行情况，请参考 Wittkowski 等人 (2019)。
