Ecossa, Starnberg, Germany.
Environ Toxicol Chem. 2012 Jul;31(7):1525-35. doi: 10.1002/etc.1843. Epub 2012 May 17.
A ring test was carried out within the standardization process of ISO 10872 to evaluate the precision of the toxicity test for the nematode Caenorhabditis elegans. Eight different laboratories tested aqueous solutions of the reference substance benzylcetyldimethylammonium chloride as well as native sediments and soils for toxic effects on the growth and reproduction of C. elegans. Validity criteria were met in all laboratories. Average median- and low-effect concentrations were determined to be 15.1 mg L(-1) (EC50) and 8.7 mg L(-1) (EC10) for growth and 7.5 mg L(-1) (EC50) and 3.8 mg L(-1) (EC10) for reproduction of C. elegans, with ECx values showing a high degree of reproducibility (CV(R) : <21% and <11% for EC10 and EC50, respectively) and repeatability (CV(r) : <20% and <7% for EC10 and EC50, respectively). The toxic effects of the sediments and soils revealed by the different laboratories were well related to each samples' degree of chemical contamination. Moreover, the effects showed an acceptable reproducibility (CV(R) : 5-33% and 0-28% for growth and reproduction, respectively) and repeatability (CV(r) : 3-13% and 0-12% for growth and reproduction, respectively). The present study confirms that the toxicity test with C. elegans according to ISO 10872 is a reliable and precise tool to assess the toxicity of aqueous media, freshwater sediments, and soils.
环试验是在 ISO 10872 的标准化过程中进行的，以评估线虫秀丽隐杆线虫毒性试验的精密度。八个不同的实验室测试了苄基椰油二甲基铵氯化物的参考物质的水溶液以及天然沉积物和土壤对秀丽隐杆线虫生长和繁殖的毒性作用。所有实验室均符合有效性标准。确定了平均中值和低效应浓度，对于生长，分别为 15.1 毫克/升（EC50）和 8.7 毫克/升（EC10），对于繁殖，分别为 7.5 毫克/升（EC50）和 3.8 毫克/升（EC10），ECx 值具有高度的重现性（EC10 和 EC50 的 CV（R）分别<21%和<11%）和可重复性（EC10 和 EC50 的 CV（r）分别<20%和<7%）。不同实验室揭示的沉积物和土壤的毒性作用与每个样品的化学污染程度密切相关。此外，该效果具有可接受的重现性（生长和繁殖的 CV（R）分别为 5-33%和 0-28%）和可重复性（生长和繁殖的 CV（r）分别为 3-13%和 0-12%）。本研究证实，根据 ISO 10872 进行的秀丽隐杆线虫毒性试验是评估水介质、淡水沉积物和土壤毒性的可靠且精确的工具。
