Department of Plant Microbe Interactions; Max Plank Institute for Plant Breeding Research, 50829 Cologne, Germany.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 113-8657 Tokyo, Japan.
STAR Protoc. 2020 Dec 15;1(3):100226. doi: 10.1016/j.xpro.2020.100226. eCollection 2020 Dec 18.
We present a gnotobiotic system for microbiota reconstitution on under contrasting iron availability. This system induces iron starvation in plants by providing an unavailable form, mimicking conditions in alkaline soils. Inoculation of taxonomically diverse bacteria reconstitutes plants with a synthetic microbiota, allowing observation of nutrient-dependent interactions with commensals. Experimental optimization, including media composition and preparation of seedlings and bacteria, is discussed. This system provides a framework that can be adapted to study plant-microbiota interactions in further nutritional contexts. For complete details on the use and execution of this protocol, please refer to Harbort et al. (2020).
我们提出了一种在不同铁供应条件下重建微生物组的无菌动物系统。该系统通过提供一种不可用的形式来诱导植物缺铁，模拟碱性土壤中的条件。接种分类上多样化的细菌可以用合成微生物组重建植物，从而可以观察到与共生菌的营养依赖性相互作用。讨论了实验优化，包括培养基组成和幼苗及细菌的制备。该系统提供了一个可以适应进一步营养环境中研究植物-微生物组相互作用的框架。有关该方案使用和执行的完整详细信息，请参见 Harbort 等人。（2020 年）。
