伊氏锥虫蛋白被携带有微丝蚴犬的个体的抗体所识别。
Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs.
机构信息
División Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México.
Laboratorio de Bioquímica y Biología Molecular, Centro de Investigaciones Científicas de Yucatán (CICY), Mérida, Yucatán, México.
出版信息
J Infect Dev Ctries. 2020 Dec 31;14(12):1442-1447. doi: 10.3855/jidc.12711.
INTRODUCTION
Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins.
METHODOLOGY
Eight serum samples from the owners of microfilaremic dogs were examined. Total proteins were extracted from adult worms and 12% SDS-PAGE was performed. The gel was electroblotted to a nitrocellulose membrane, and a Western blot (WB) was performed. Reactive bands of 22, 33, 39, 49, and 63 kDa in WB were excised from the gel and analyzed by mass spectrometry (MS).
RESULTS
The MS results showed the presence of 10 different proteins of D. immitis recognized by the human serum samples.
CONCLUSIONS
These results indicate that in endemic areas of D. immitis, owners of infected dogs recognize specific proteins of the parasite, suggesting a possible infection.
简介
犬恶丝虫是一种影响世界上多个国家人类健康的线虫。本研究旨在检测微丝蚴血症犬主人的血清样本是否对寄生虫蛋白具有免疫反应性。
方法
检测了 8 份微丝蚴血症犬主人的血清样本。从成虫中提取总蛋白，并进行 12% SDS-PAGE。将凝胶电转移至硝酸纤维素膜上，进行 Western blot（WB）。从 WB 中切下 22、33、39、49 和 63 kDa 的反应带，并通过质谱（MS）进行分析。
结果
MS 结果显示，人血清样本识别出犬恶丝虫的 10 种不同蛋白。
结论
这些结果表明，在犬恶丝虫流行地区，感染犬的主人能够识别寄生虫的特定蛋白，提示可能存在感染。
Zotero 插件