Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA; Department of Biochemistry, Emory University, Atlanta, GA 30322, USA.
Cell Chem Biol. 2021 Apr 15;28(4):567-582.e4. doi: 10.1016/j.chembiol.2020.12.005. Epub 2020 Dec 29.
The pleiotropic functions of macrophages in immune defense, tissue repair, and maintenance of tissue homeostasis are supported by the heterogeneity in macrophage sub-populations that differ both in ontogeny and polarization. Although glycans and glycan-binding proteins (GBPs) are integral to macrophage function and may contribute to macrophage diversity, little is known about the factors governing their expression. Here, we provide a resource for characterizing the N-/O-glycomes of various murine peritoneal macrophage sub-populations, demonstrating that glycosylation primarily reflects developmental origin and, to a lesser degree, cellular polarization. Furthermore, comparative analysis of GBP-coding genes in resident and elicited macrophages indicated that GBP expression is consistent with specialized macrophage functions and correlates with specific types of displayed glycans. An integrated, semi-quantitative approach was used to confirm distinct expression patterns of glycans and their binding proteins across different macrophages. The data suggest that regulation of glycan-protein complexes may be central to macrophage residence and recruitment.
巨噬细胞在免疫防御、组织修复和组织稳态维持中的多种功能是由巨噬细胞亚群的异质性支持的，这些亚群在个体发生和极化方面存在差异。尽管聚糖和糖结合蛋白（GBP）是巨噬细胞功能的组成部分，并且可能有助于巨噬细胞的多样性，但关于控制它们表达的因素知之甚少。在这里，我们提供了一个资源，用于描述各种小鼠腹腔巨噬细胞亚群的 N-/O-聚糖组，表明糖基化主要反映了发育起源，在较小程度上反映了细胞极化。此外，对常驻和募集巨噬细胞中 GBP 编码基因的比较分析表明，GBP 的表达与巨噬细胞的特定功能一致，并与所显示的特定类型的聚糖相关。采用集成的半定量方法来确认不同巨噬细胞中聚糖及其结合蛋白的不同表达模式。这些数据表明，糖蛋白复合物的调节可能是巨噬细胞驻留和募集的核心。
