Bezdekova Jaroslava, Vodova Milada, Dolezelikova Kristyna, Zitka Jan, Smerkova Kristyna, Zitka Ondrej, Adam Vojtech, Vaculovicova Marketa
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic.
Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic.
Talanta. 2021 Mar 1;224:121813. doi: 10.1016/j.talanta.2020.121813. Epub 2020 Oct 28.
The here presented work is focused on the development of a method for detection of microbial contamination of food based on uracil-selective synthetic receptors. Because uracil may serve as an indicator of bacterial contamination, its selective and on-site detection may prevent spreading of foodborne diseases. The synthetic receptors were created by molecular imprinting. Molecularly imprinted polymers for selective uracil isolation were prepared by a non-covalent imprinting method using dopamine as a functional monomer. Detection of isolated uracil was performed by capillary electrophoresis with absorption detection (λ - 260 nm). The conditions of preparation of molecularly imprinted polymers, their binding properties, adsorption kinetics and selectivity were investigated in detail. Furthermore, the prepared polymer materials were used for selective isolation and detection of uracil from complex samples as tomato products by miniaturized electrophoretic system suggesting the potential of in situ analysis of real samples.
本文所展示的工作聚焦于基于尿嘧啶选择性合成受体开发一种检测食品微生物污染的方法。由于尿嘧啶可作为细菌污染的指标，其选择性和现场检测可预防食源性疾病的传播。合成受体通过分子印迹法制备。使用多巴胺作为功能单体，通过非共价印迹法制备了用于选择性分离尿嘧啶的分子印迹聚合物。通过毛细管电泳吸收检测（λ = 260 nm）对分离出的尿嘧啶进行检测。详细研究了分子印迹聚合物的制备条件、结合性能、吸附动力学和选择性。此外，所制备的聚合物材料通过小型化电泳系统用于从番茄制品等复杂样品中选择性分离和检测尿嘧啶，表明了对实际样品进行原位分析的潜力。
