Feng Sinuo, Yu Linying, Yan Mengxia, Ye Jing, Huang Jianshe, Yang Xiurong
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China; University of Science and Technology of China, Hefei, Anhui, 230026, China.
University of Science and Technology of China, Hefei, Anhui, 230026, China.
Talanta. 2021 Mar 1;224:121851. doi: 10.1016/j.talanta.2020.121851. Epub 2020 Nov 5.
In this paper, holey nitrogen-doped graphene aerogel (HNGA) was synthesized and applied to the concurrently electrochemical determination of small biological molecules including ascorbic acid (AA), dopamine (DA) and uric acid (UA). Firstly, holey graphene hydrogel was synthesized by the hydrothermal reaction in the presence of HO, which subsequently was lyophilized and further annealed in the mixed gas of ammonia and argon to obtain HNGA. Electron microscopy characterization exhibited a great number of nanopores formed on the basal surface of graphene sheets, and HNGA possessed a hierarchically porous structure. The unique structure and composition of HNGA make it an ideal material for electroanalytical application through accelerating mass and electron transfer. HNGA modified glassy carbon electrode (HNGA/GCE) displayed significantly enhanced electrochemical response to AA, DA, and UA, namely reducing overpotential, increasing current density, and improving the reversibility. The oxidation peaks of these three biomolecules can be entirely separated with evident peak potential differences which are 0.216 V (AA-DA), 0.120 V (DA-UA), and 0.336 V (AA-UA), which it allowed the determination of the three substances at the same time. This sensor shows high sensitivity for the determination of AA, DA, and UA with the detection limit of 16.7 μM, 0.22 μM, and 0.12 μM (S/N = 3), respectively. The proposed sensor was applicable for the practical sample analysis as well and desirable recovery was obtained.
本文合成了多孔氮掺杂石墨烯气凝胶（HNGA），并将其应用于同时电化学测定包括抗坏血酸（AA）、多巴胺（DA）和尿酸（UA）在内的小生物分子。首先，在过氧化氢存在下通过水热反应合成多孔石墨烯水凝胶，随后将其冻干并在氨和氩的混合气体中进一步退火以获得HNGA。电子显微镜表征显示在石墨烯片的基面形成了大量纳米孔，且HNGA具有分级多孔结构。HNGA独特的结构和组成使其成为通过加速质量和电子转移进行电分析应用的理想材料。HNGA修饰玻碳电极（HNGA/GCE）对AA、DA和UA表现出显著增强的电化学响应，即降低过电位、增加电流密度并提高可逆性。这三种生物分子的氧化峰可以完全分离，具有明显的峰电位差，分别为0.216 V（AA - DA）、0.120 V（DA - UA）和0.336 V（AA - UA），这使得能够同时测定这三种物质。该传感器对AA、DA和UA的测定具有高灵敏度，检测限分别为16.7 μM、0.22 μM和0.12 μM（S/N = 3）。所提出的传感器也适用于实际样品分析，并获得了理想的回收率。
