Department of Chemistry, Faculty of Mathematics and Natural Sciences, Indonesia Defense University, Kawasan IPSC Sentul, Bogor, 16810, Indonesia.
Analytical Chemistry Division, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Kampus IPB Dramaga, Bogor, 16680, Indonesia.
Anal Sci. 2022 Jan;38(1):157-166. doi: 10.2116/analsci.21P214. Epub 2022 Feb 28.
A glassy carbon electrode modified with electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) was developed for uric acid determination with dopamine as interference in artificial saliva. The electrochemical performance of the fabricated electrode was studied using both techniques of cyclic voltammetry (CV) and differential pulse voltammetry (DPV), under optimized conditions. Using DPV, the sensor based on ErGO/PEDOT:PSS modified GCE displayed a linear relationship in the concentration ranges of 10-100 µM for uric acid. This uric acid sensor exhibited a high sensitivity with detection limits of 1.08 µM and quantitation limit of 3.61 µM. This sensor also showed good reproducibility for uric acid detection in artificial saliva in 5 consecutive days of measurements. This device was successfully used to analyze uric acid in artificial saliva as well as in a human saliva sample using the standard addition method.
一种玻碳电极，经过电化学还原氧化石墨烯（ErGO）和聚（3,4-乙二氧基噻吩）：聚（苯乙烯磺酸盐）（PEDOT：PSS）修饰，用于在人工唾液中存在多巴胺干扰的情况下测定尿酸。在优化条件下，使用循环伏安法（CV）和差分脉冲伏安法（DPV）两种技术研究了所制备电极的电化学性能。使用 DPV，基于 ErGO/PEDOT：PSS 修饰的 GCE 的传感器在尿酸的浓度范围为 10-100µM 时显示出线性关系。这种尿酸传感器具有高灵敏度，检测限为 1.08µM，定量限为 3.61µM。该传感器在连续 5 天的测量中对人工唾液中的尿酸检测也表现出良好的重现性。该装置成功地使用标准添加法分析了人工唾液和人类唾液样本中的尿酸。
