Qiu Bin, Li Guangyong, Du Jianke, Zhang Aibing, Jin Yuan
Smart Materials and Advanced Structure Laboratory, School of Mechanical Engineering and Mechanics, Ningbo University, Ningbo 315211, China.
Materials (Basel). 2020 Dec 28;14(1):95. doi: 10.3390/ma14010095.
A few simple polymeric microsystems, such as microcantilever sensors, have recently been developed for the preliminary screening of cardiac toxicity. The microcantilever deflection produced by a change in the cardiomyocyte (CM) contraction force is important for understanding the mechanism of heart failure. In this study, a new numerical model is proposed to analyze the contractile behavior of CMs cultured on a perforated microcantilever surface for improving the performance of the microcantilever sensor. First, the surface traction model is used to investigate the bending displacement of the plain microcantilever. In order to improve the bending effect, a new numerical model is developed to analyze the bending behavior of the perforated microcantilever covered with CMs. Compared with the designed molds, the latter yields better results. Finally, a simulation analysis is proposed based on a finite element method to verify the presence of a preformed mold. Moreover, the effects of various factors on the bending displacement, including microcantilever size, Young's modulus, and porosity factor, are investigated. Both the simulation and numerical results have good consistency, and the maximum error between the numerical and simulation results is not more than 3.4%, even though the porosity factor reaches 0.147. The results show that the developed mold opens new avenues for CM microcantilever sensors to detect cardiac toxicity.
最近已经开发出一些简单的聚合物微系统，如微悬臂梁传感器，用于心脏毒性的初步筛查。心肌细胞（CM）收缩力变化产生的微悬臂梁挠度对于理解心力衰竭机制很重要。在本研究中，提出了一种新的数值模型来分析培养在多孔微悬臂梁表面的心肌细胞的收缩行为，以提高微悬臂梁传感器的性能。首先，使用表面牵引力模型研究普通微悬臂梁的弯曲位移。为了提高弯曲效果，开发了一种新的数值模型来分析覆盖有心肌细胞的多孔微悬臂梁的弯曲行为。与设计的模具相比，后者产生了更好的结果。最后，基于有限元方法提出了模拟分析，以验证预制模具的存在。此外，研究了各种因素对弯曲位移的影响，包括微悬臂梁尺寸、杨氏模量和孔隙率因子。模拟结果和数值结果具有良好的一致性，即使孔隙率因子达到0.147，数值结果与模拟结果之间的最大误差也不超过3.4%。结果表明，所开发的模具为心肌细胞微悬臂梁传感器检测心脏毒性开辟了新途径。
