Kim Dae Jung, Weeks Brandon L, Hope-Weeks Louisa J
Department of Chemistry and Biochemistry, Texas Tech University, Texas 79409-1061, USA.
Scanning. 2007 Nov-Dec;29(6):245-8. doi: 10.1002/sca.20076.
Microcantilever sensors are an offshoot of atomic force microscopy and are useful tools for effectively detecting a target biomolecule. The recognition of the target molecule on the biosensor is based on the physical bending of the microcantilever, which is driven by a specific molecular interaction between the target molecule and the sensor surface. In this study, to enhance the sensitivity of the microcantilever sensor, the sensor surface was modified through a surface conjugation method using self-assembled monolayers (SAMs) and heterobifunctional cross-linkers. After the surface modification of the microcantilever sensor, the sensitivity for L-cysteine was recorded. The detection of L-cysteine was influenced by the active site and the molecular size of the cross-linked compound attached onto the surface of the microcantilever.
微悬臂梁传感器是原子力显微镜的衍生物，是有效检测目标生物分子的有用工具。生物传感器上目标分子的识别基于微悬臂梁的物理弯曲，这是由目标分子与传感器表面之间的特定分子相互作用驱动的。在本研究中，为提高微悬臂梁传感器的灵敏度，通过使用自组装单分子层（SAMs）和异双功能交联剂的表面共轭方法对传感器表面进行了修饰。对微悬臂梁传感器进行表面修饰后，记录了其对L-半胱氨酸的灵敏度。微悬臂梁表面连接的交联化合物的活性位点和分子大小影响了L-半胱氨酸的检测。
