Anuragi Hirdayesh, Dhaduk Haresh L, Kumar Sushil, Dhruve Jitendra J, Parekh Mithil J, Sakure Amar A
Department of Genetics and Plant Breeding, B. A. College of Agriculture, Anand Agricultural University, Anand, Gujarat, 388 110, India.
Department of Agricultural Biotechnology, Centre of Excellence in Biotechnology, Anand Agricultural University, Anand, Gujarat, 388 110, India.
3 Biotech. 2016 Dec;6(2):204. doi: 10.1007/s13205-016-0520-9. Epub 2016 Sep 23.
Understanding the genetic variation in germplasm is of utmost importance for crop improvement. Therefore, efforts were made to analyse the molecular marker based genetic diversity of 20 Annona genotypes from five different species of family Annonaceae. During analysis, a set of 11 RAPD primers yielded a total of 152 bands with 80.01 % polymorphism and PIC for RAPD ranged from 0.86 to 0.92 with a mean of 0.89. With 93.05 % polymorphism, 12 SSR primers produced 39 amplicons. The PIC for SSRs ranged from 0.169 to 0.694 with of average of 0.339. The dendrogram produced from pooled molecular data of 11 RAPD and 12 SSR primers showed seven clusters at a cutoff value of 0.78. The dendrogram discriminated all the Annona genotypes suggesting that significant genetic diversity was present among the genotypes. Proximate fruit composition study of nine fruiting genotypes of Annona revealed that A. squamosa possessed significantly higher amount of most of studies biochemical which gives an opportunity to fruit breeders to improve the other Annona species. Likewise, A. muricata being rich in seed oil content can be exploited in oil industries.
了解种质资源中的遗传变异对于作物改良至关重要。因此，我们努力分析了番荔枝科五个不同物种的20个番荔枝基因型基于分子标记的遗传多样性。在分析过程中，一组11个RAPD引物共产生了152条带，多态性为80.01%，RAPD的PIC值在0.86至0.92之间，平均值为0.89。12个SSR引物产生了39个扩增子，多态性为93.05%。SSR的PIC值在0.169至0.694之间，平均值为0.339。由11个RAPD和12个SSR引物的合并分子数据生成的聚类图在截止值为0.78时显示出七个聚类。聚类图区分了所有番荔枝基因型，表明基因型之间存在显著的遗传多样性。对九个结果番荔枝基因型的近似果实成分研究表明，番荔枝的大部分研究生化成分含量显著更高，这为果实育种者提供了改良其他番荔枝物种的机会。同样，刺果番荔枝富含种子油，可以在油脂工业中加以利用。
