Rutten A A, Jongen W M, de Haan L H, Hendriksen E G, Koeman J H
TNO-CIVO Toxicology and Nutrition Institute, Department of Biological Toxicology, Zeist, The Netherlands.
Carcinogenesis. 1988 Feb;9(2):315-20. doi: 10.1093/carcin/9.2.315.
The dye-coupled intercellular communication across gap junctions in primary hamster tracheal epithelial cells has been studied in serum-free, hormone-supplemented medium. In the absence of vitamin A, non-cytotoxic concentrations of cigarette-smoke condensate (CSC) inhibited intercellular communication between tracheal epithelial cells in a concentration-dependent way. All-trans retinol and retinoic acid showed biphasic effects on intercellular communication depending on their concentration. Physiological concentrations of retinol and retinoic acid increased the dye-coupled transfer of Lucifer Yellow CH via gap junctions compared with the dimethylsulfoxide-treated tracheal epithelial cells. At pharmacological concentrations retinol slightly increased the intercellular communication in the first 2 h of the exposure period, whereas upon longer treatment times with retinol and retinoic acid, gap-junction-mediated intercellular communication was inhibited almost completely. When retinol was given to tracheal epithelial cells before exposure to CSC or simultaneously with CSC-exposure, retinol counteracted the inhibitory potential of CSC on intercellular communication. The results of the present study clearly indicate that both CSC and all-trans retinol influence the intercellular communication between primary hamster tracheal epithelial cells in serum-free, hormone-supplemented culture medium.
在无血清、添加激素的培养基中，对原代仓鼠气管上皮细胞间通过间隙连接进行的染料偶联细胞间通讯进行了研究。在缺乏维生素A的情况下，无细胞毒性浓度的香烟烟雾冷凝物（CSC）以浓度依赖的方式抑制气管上皮细胞间的细胞间通讯。全反式视黄醇和视黄酸对细胞间通讯表现出双相效应，这取决于它们的浓度。与用二甲基亚砜处理的气管上皮细胞相比，生理浓度的视黄醇和视黄酸通过间隙连接增加了荧光素黄CH的染料偶联转移。在药理学浓度下，视黄醇在暴露期的前2小时略微增加了细胞间通讯，而在用视黄醇和视黄酸进行更长时间处理后，间隙连接介导的细胞间通讯几乎完全被抑制。当在暴露于CSC之前或与CSC同时给予气管上皮细胞视黄醇时，视黄醇抵消了CSC对细胞间通讯的抑制作用。本研究结果清楚地表明，CSC和全反式视黄醇均影响无血清、添加激素的培养基中原代仓鼠气管上皮细胞间的细胞间通讯。
