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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Corfield A P, Williams A J, Clamp J R, Wagner S A, Mountford R A

University Department of Medicine Laboratories, Bristol Royal Infirmary, U.K.

Clin Sci (Lond). 1988 Jan;74(1):71-8. doi: 10.1042/cs0740071.

The activities of enzymes degrading human colonic mucin were examined in faecal specimens from healthy subjects and patients with inflammatory bowel disease. 2. The activity of sialidase was measured using a new physiological substrate related to mucus glycoproteins. In addition, acylneuraminate pyruvate-lyase (N-acetylneuraminate lyase; EC 4.1.3.3.) and a novel O-acetylsialic acid esterase (sialate O-acetylesterase; EC 3.1.1.53) were detected. 3. The O-acetylsialic acid esterase activity was readily detectable in partially purified fractions after Sephadex G-100 chromatography. 4. Patients with inflammatory bowel disease showed significant increases in acylneuraminate pyruvate-lyase and proteinase activity but sialidase activity did not differ from normal. The activity of these enzymes in neutrophils could not account for the differences observed.

对健康受试者和炎症性肠病患者粪便样本中降解人结肠黏蛋白的酶活性进行了检测。2. 使用一种与黏液糖蛋白相关的新型生理底物来测定唾液酸酶的活性。此外，还检测到了酰基神经氨酸丙酮酸裂解酶（N - 乙酰神经氨酸裂解酶；EC 4.1.3.3.）和一种新型的O - 乙酰唾液酸酯酶（唾液酸O - 乙酰酯酶；EC 3.1.1.53）。3. 在经Sephadex G - 100层析后的部分纯化组分中很容易检测到O - 乙酰唾液酸酯酶活性。4. 炎症性肠病患者的酰基神经氨酸丙酮酸裂解酶和蛋白酶活性显著增加，但唾液酸酶活性与正常情况无差异。中性粒细胞中这些酶的活性无法解释所观察到的差异。

Corfield A P, Williams A J, Clamp J R, Wagner S A, Mountford R A

University Department of Medicine Laboratories, Bristol Royal Infirmary, U.K.

Clin Sci (Lond). 1988 Jan;74(1):71-8. doi: 10.1042/cs0740071.

The activities of enzymes degrading human colonic mucin were examined in faecal specimens from healthy subjects and patients with inflammatory bowel disease. 2. The activity of sialidase was measured using a new physiological substrate related to mucus glycoproteins. In addition, acylneuraminate pyruvate-lyase (N-acetylneuraminate lyase; EC 4.1.3.3.) and a novel O-acetylsialic acid esterase (sialate O-acetylesterase; EC 3.1.1.53) were detected. 3. The O-acetylsialic acid esterase activity was readily detectable in partially purified fractions after Sephadex G-100 chromatography. 4. Patients with inflammatory bowel disease showed significant increases in acylneuraminate pyruvate-lyase and proteinase activity but sialidase activity did not differ from normal. The activity of these enzymes in neutrophils could not account for the differences observed.

对健康受试者和炎症性肠病患者粪便样本中降解人结肠黏蛋白的酶活性进行了检测。2. 使用一种与黏液糖蛋白相关的新型生理底物来测定唾液酸酶的活性。此外，还检测到了酰基神经氨酸丙酮酸裂解酶（N - 乙酰神经氨酸裂解酶；EC 4.1.3.3.）和一种新型的O - 乙酰唾液酸酯酶（唾液酸O - 乙酰酯酶；EC 3.1.1.53）。3. 在经Sephadex G - 100层析后的部分纯化组分中很容易检测到O - 乙酰唾液酸酯酶活性。4. 炎症性肠病患者的酰基神经氨酸丙酮酸裂解酶和蛋白酶活性显著增加，但唾液酸酶活性与正常情况无差异。中性粒细胞中这些酶的活性无法解释所观察到的差异。