来自粟酒裂殖酵母的酪氨酸可抑制的3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶的纯化。
Purification of the tyrosine inhibitable 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Schizosaccharomyces pombe.
作者信息
Bracher M, Schweingruber E
出版信息
Biochim Biophys Acta. 1977 Dec 8;485(2):446-51. doi: 10.1016/0005-2744(77)90180-2.
PMID:21700
Abstract
A method is described for the purification of the tyrosine inhibitable isoenzyme 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase(pyruvate phosphorylating), EC 4.1.2.15) to homogeneity as judged by polyacrylamide gel electrophoresis.
摘要
本文描述了一种纯化酪氨酸可抑制的同工酶3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶（7-磷酸-2-酮-3-脱氧-D-阿拉伯庚酮酸-D-赤藓糖-4-磷酸裂解酶（丙酮酸磷酸化），EC 4.1.2.15）的方法，通过聚丙烯酰胺凝胶电泳判断，该方法可将其纯化至同质状态。
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