Department of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University, Doha 2713, Qatar.
Int J Environ Res Public Health. 2020 Dec 29;18(1):182. doi: 10.3390/ijerph18010182.
This research was conducted to investigate the dynamics of airborne fungi using viable culture collection and in respect to different abiotic variables, including seasonal and intra-diurnal variations. A gravimetric method was used to sample airborne fungal deposition on potato dextrose agar plates on alternate days, for a year between April 2015 to March 2016. From 176 settle plate exposures, a total of 1197 mould and 283 yeast colony-forming units (CFU), 21 genera and 62 species were retrieved. The highest fungal spore count was recorded in February 2016, whereas the lowest count occurred in August 2015. The main constituents of the fungal airspora were attributed to (60.2%), (10.4%), (9.4%), (8.5%), and spp. (2.3%). Temperature was negatively correlated with total colony count ( = -0.231, ≤ 0.05) or species richness ( = -0.267, ≤ 0.001), while wind speed was positively correlated with total colony count ( = 0.484, ≤ 0.001) or species richness ( = 0.257, ≤ -0.001). The highest dispersal of fungal spores was obtained at 18:00, whereas the lowest fungal spores release was recorded at 00:00 (midnight). There were no significant differences in species composition and richness of the airborne fungal population between two study sites, the Industrial area and Qatar University Campus. The count of spp. and spp. were significantly higher at the Industrial area site, which corresponds to a higher CO level than the Qatar University site. This study lays the foundation for future work to assess the implications of such aeromycological data on public health.
本研究旨在通过可培养的生物收集方法，研究空气真菌的动态变化，并结合不同的非生物变量（包括季节性和日内变化）进行分析。研究采用重力法，在 2015 年 4 月至 2016 年 3 月的一年内，每天交替在土豆葡萄糖琼脂平板上采样空气中真菌的沉降量。在 176 次沉降平板暴露实验中，共回收了 1197 个霉菌和 283 个酵母落形成单位（CFU），涉及 21 属和 62 种。2016 年 2 月的真菌孢子计数最高，而 2015 年 8 月的计数最低。空气真菌群落的主要成分归因于 （60.2%）、 （10.4%）、 （9.4%）、 （8.5%）和 spp.（2.3%）。温度与总菌落计数（ = -0.231， ≤ 0.05）或物种丰富度（ = -0.267， ≤ 0.001）呈负相关，而风速与总菌落计数（ = 0.484， ≤ 0.001）或物种丰富度（ = 0.257， ≤ -0.001）呈正相关。真菌孢子的最高扩散量是在 18:00 获得的，而最低的真菌孢子释放是在 00:00（午夜）记录的。两个研究地点（工业区和卡塔尔大学校园）的空气真菌种群的物种组成和丰富度没有显著差异。在工业区地点， spp.和 spp.的数量明显较高，这与该地点的 CO 水平高于卡塔尔大学地点相对应。本研究为未来评估此类空气微生物数据对公共健康的影响奠定了基础。
