Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Hanzeplein 1 EB80, 9713 GZ Groningen, The Netherlands.
Department of Pediatrics, University of Groningen, University Medical Center Groningen, Hanzeplein 1 EB80, 9713 GZ Groningen, The Netherlands.
Biomolecules. 2020 Dec 29;11(1):34. doi: 10.3390/biom11010034.
Mucositis is a common side-effect of chemotherapy treatment, inducing alterations in the composition of the gut microbiota. Redox active compounds, such as vitamins B2 and C, have been shown to reduce inflammation and enhance the growth of anaerobic bacteria in the gut. We therefore aimed to (1) validate the ability of these compounds to promote bacterial cell growth in vitro, and (2) determine their prophylactic efficacy in a rat model of methotrexate (MTX)-induced mucositis. Bacterial growth curves were performed to assess the growth kinetics of bacteria exposed to Vitamins C and B2 (0.5 mM). Male wistar rats (150-200 g) received vitamins B2 (12 mg/day) and C (50 mg/day) via daily oral gavage (from day -1 to day 10). MTX (45 mg/Kg) was administrated via I.V. injection (N = 4-8/group) on day 0. Body weight, water/food consumption and diarrhea were assessed daily. Blood and faecal samples were collected longitudinally to assess citrulline levels (mucositis biomarker) and gut microbiota composition. Vitamins C/B2 enhanced the in vitro growth of anaerobic bacteria and . Contrarily to vitamin B2, in vivo administration of Vitamin C significantly attenuated clinical symptoms of mucositis. Despite their influence on the composition of the gut microbiota, both vitamins did not modulate the course of MTX-induced mucositis, as accessed by plasma citrulline. Vitamins B2 and C enhanced anaerobic bacterial growth in vitro, however their ability to mitigate MTX-induced mucositis was limited.
黏膜炎是化疗治疗的常见副作用，会引起肠道微生物组组成的改变。氧化还原活性化合物，如维生素 B2 和 C，已被证明可以减轻炎症并促进肠道中厌氧菌的生长。因此，我们旨在：(1)验证这些化合物在体外促进细菌细胞生长的能力，(2)确定它们在甲氨蝶呤 (MTX) 诱导的黏膜炎大鼠模型中的预防效果。通过细菌生长曲线评估暴露于维生素 C 和 B2（0.5 mM）的细菌的生长动力学。雄性 Wistar 大鼠（150-200 g）通过每日口服灌胃（从第-1 天到第 10 天）接受维生素 B2（12 mg/天）和 C（50 mg/天）。MTX（45 mg/Kg）通过 IV 注射（每组 4-8 只）在第 0 天给药。每日评估体重、水/食物消耗和腹泻情况。收集血液和粪便样本以纵向评估瓜氨酸水平（黏膜炎生物标志物）和肠道微生物组组成。维生素 C/B2 增强了厌氧细菌的体外生长。与维生素 B2 相反，维生素 C 的体内给药显著减轻了黏膜炎的临床症状。尽管它们对肠道微生物组的组成有影响，但两种维生素都没有通过血浆瓜氨酸来调节 MTX 诱导的黏膜炎的进程。维生素 B2 和 C 增强了体外厌氧细菌的生长，但它们减轻 MTX 诱导的黏膜炎的能力有限。
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