Zuo Lingli, Zhou Liting, Wu Chaoyi, Wang Yanlin, Li Yuanyuan, Huang Rui, Wu Shuyan
Department of Medical Microbiology, School of Biology and Basic Medical Science, Medical College of Soochow University, Suzhou, China.
Medical Research Center, The People's Hospital of Suzhou New District, Suzhou, China.
Front Microbiol. 2020 Dec 15;11:562491. doi: 10.3389/fmicb.2020.562491. eCollection 2020.
serovar Typhimurium (). Typhimurium is a primary foodborne pathogen infecting both humans and animals. plasmid virulence C () gene is closely related to . Typhimurium dissemination in mice, while the mechanisms remain to be fully elucidated. Pyroptosis, a gasdermin-mediated inflammatory cell death, plays a role in host defense against bacterial infection, whereas the effect of on pyroptosis and its function in inflammatory injury induced by . Typhimurium are rather limited. In our study, C57BL/6 mice and J774A.1 cells infected with . Typhimurium wild-type strain SL1344, deletion mutant, K136A site-directed mutant, and complemented strain were used to investigate potential pathogenesis of . We verity that SpvC attenuates intestinal inflammation, suppresses pyroptosis through phosphothreonine lyase activity, and reduces pyroptosis in the ceca. Moreover, the reduction of inflammation via results in systemic infection. These findings demonstrate that inhibits pyroptosis and intestinal inflammation to promote bacterial dissemination, which provide new strategies for controlling systemic infection caused by and novel insights for the treatment of other corresponding diseases.
鼠伤寒血清型（）。鼠伤寒是一种主要的食源性病原体，可感染人类和动物。质粒毒力C（）基因与鼠伤寒在小鼠体内的传播密切相关，但其机制仍有待充分阐明。细胞焦亡是一种由gasdermin介导的炎性细胞死亡，在宿主抵抗细菌感染中发挥作用，而关于对细胞焦亡的影响及其在鼠伤寒诱导的炎性损伤中的作用却相当有限。在我们的研究中，使用感染了鼠伤寒野生型菌株SL1344、缺失突变体、K136A定点突变体和互补菌株的C57BL/6小鼠和J774A.1细胞来研究鼠伤寒的潜在发病机制。我们证实SpvC可减轻肠道炎症，通过磷酸苏氨酸裂解酶活性抑制细胞焦亡，并减少盲肠中的细胞焦亡。此外，通过导致的炎症减轻会引发全身感染。这些发现表明抑制细胞焦亡和肠道炎症以促进细菌传播，这为控制由引起的全身感染提供了新策略，并为治疗其他相应疾病提供了新见解。
