Relationship between expression mRNA gene Treg, Treg, CD4, and CD8 protein levels with TST in tuberculosis children: A nested case-control.

BACKGROUND

The ability of to survive intracellularly, provides a cellular adaptive immune response played by specific T cells to defend against tuberculosis. The adaptive immune response to Bacillus of Calmette and Guerin (BCG) immunization is responded to by B cells, T Follicular B helper, T regulatory, restriction CD1, CD8, CD4, Th1, Th2, and Th17. BCG immunization can cause a tuberculin test reaction to being positive. The tuberculin test is a method for diagnosing TB infection and for screening individuals for latent infection and assessing the rate of TB infection in a given population.

METHODS

a nested case-control survey was conducted on patients with a diagnosis of TB and parents 0-18 years of age from 3 hospitals in Indonesia during September-November 2019 with a total sample of 69 people undergoing clinical examinations, supporting and diagnosing subjects, blood sampling 1-2 cc for examination mRNA gene Treg, Treg, CD 4+, and CD 8+, then centrifuged at 3000 rpm for 10 min to support blood cells and serum.

RESULTS

There was a significant relationship between expression of mRNA gene Treg with TST (p = 0,000), Treg with TST (p = 0,000), and CD4 with TST (p = 0,000). Meanwhile, CD8 + was not significantly associated with TST (p = 0.118).

CONCLUSIONS

It is necessary to check the expression of mRNA gene Treg, Treg, CD4, and CD8 with more samples to find the mean value that shows the protective value of further TB.