Kurniawan Aryadi, Kodrat Evelina, Gani Yogi Ismail
Paediatric Orthopaedic Division, Department of Orthopaedics and Traumatology, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Musculoskletal Pathology Division, Departement of Anatomic Phatology, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo Hospital, Jakarta, Indonesia.
Ann Med Surg (Lond). 2020 Dec 17;61:54-60. doi: 10.1016/j.amsu.2020.12.005. eCollection 2021 Jan.
Delayed union is a problem that can occur after fracture healing. Many studies were conducted based on the diamond concept approach to solve the problem of delayed union. Granulocyte-colony stimulating factor (G-CSF) is one of the various substances known to have a positive role in healing skeletal tissue or adjuvant regeneration. This study was conducted to see the effect of G-CSF in affecting delayed union fracture healing.
The experimental study was conducted by randomized posttest only control group design on 24 experimental animals Sprague-Dawley white rats that had experienced delayed union models. The study compared the treatment group injected with subcutaneous G-CSF with a control group and was divided into four groups (n = 6). Harvest and follow-up histomorphometry and immunohistochemistry were performed in the second week and in the fourth week the histomorphometry analysis consisted of the percentage of immature bone area, cartilage, and fibrous area. The semiquantitative evaluation of immunohistochemistry with the expression of BMP-2 through the immunoreactive score (IRS).
In the evaluation of histomorphometry and immunohistochemical parameters, there were significantly more woven bone area (p = 0,015), less fibrosis area (p = 0,002) and higher BMP 2 expression (p = 0,004) in treatment group week four compared to control. .
G-CSF was shown to increase the speed of healing in Sprague-Dawley rats on delayed union models evaluated from histomorphometry and immunohistochemical aspects.
骨折愈合延迟是骨折愈合后可能出现的问题。许多研究基于钻石概念方法来解决骨折愈合延迟的问题。粒细胞集落刺激因子（G-CSF）是已知对骨骼组织愈合或辅助再生具有积极作用的多种物质之一。本研究旨在观察G-CSF对骨折愈合延迟的影响。
采用随机仅后测对照组设计，对24只经历骨折愈合延迟模型的Sprague-Dawley白色实验大鼠进行实验研究。将皮下注射G-CSF的治疗组与对照组进行比较，并分为四组（n = 6）。在第二周进行收获及随访组织形态计量学和免疫组织化学检测，在第四周进行组织形态计量学分析，包括未成熟骨面积、软骨和纤维面积的百分比。通过免疫反应评分（IRS）对BMP-2表达进行免疫组织化学半定量评估。
在组织形态计量学和免疫组织化学参数评估中，与对照组相比，治疗组在第四周时编织骨面积显著增加（p = 0.015），纤维化面积显著减少（p = 0.002），BMP 2表达显著升高（p = 0.004）。
从组织形态计量学和免疫组织化学方面评估，G-CSF可提高Sprague-Dawley大鼠骨折愈合延迟模型的愈合速度。
