Umiatin U, Hadisoebroto Dilogo Ismail, Sari Puji, Kusuma Wijaya Sastra
Department of Physics, Faculty of Mathematics and Natural Science, State University of Jakarta, Jakarta, Indonesia.
Doctoral Program in Biomedical Science, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
Scientifica (Cairo). 2021 Aug 25;2021:4791172. doi: 10.1155/2021/4791172. eCollection 2021.
Delayed union and nonunion fractures are clinical challenges for orthopedic surgeons. The development of fracture complications, such as delayed union and nonunion fractures, is still difficult to predict. Various methods are being investigated to improve fracture healing and prevent complications in patients. There are various methods to promote fracture healing, broadly divided into biological, chemical, and physical methods. One of the most widely used physical methods to promote fracture healing is the pulsed electromagnetic field (PEMF). This study aimed to evaluate the healing process of delayed union fracture after being stimulated by PEMF. Twenty-four rats were randomly divided into two groups: the control group ( = 12) and the PEMF group ( = 12). Delayed union fracture was performed on the left femur of all rats. Subsequently, the PEMF group was given PEMF stimulus with a magnetic field intensity of 1.6 mT and a frequency of 50 Hz for 4 hours/day and 7 days/week. The fracture healing process was evaluated on days 5, 10, 18, and 28 based on the bone callus histology using safranin O fast green (SOFG) staining. The results of the histological analysis showed that bone cartilage was higher in the PEMF group than in the control group throughout the observation period. In addition, the PEMF group had less fibrous tissue at the beginning of the healing. This finding indicates PEMF stimulation has an effect on inducing osteogenesis on fracture healing and reducing the risk of delayed union.
骨折延迟愈合和不愈合是骨科医生面临的临床挑战。骨折并发症如延迟愈合和不愈合的发生仍然难以预测。目前正在研究各种方法来促进骨折愈合并预防患者出现并发症。促进骨折愈合有多种方法，大致可分为生物方法、化学方法和物理方法。促进骨折愈合最广泛使用的物理方法之一是脉冲电磁场（PEMF）。本研究旨在评估PEMF刺激后延迟愈合骨折的愈合过程。将24只大鼠随机分为两组：对照组（n = 12）和PEMF组（n = 12）。对所有大鼠的左股骨造成延迟愈合骨折。随后，PEMF组给予磁场强度为1.6 mT、频率为50 Hz的PEMF刺激，每天4小时，每周7天。在第5、10、18和28天，基于番红O固绿（SOFG）染色的骨痂组织学评估骨折愈合过程。组织学分析结果表明，在整个观察期内，PEMF组的骨软骨比对照组更高。此外，PEMF组在愈合初期的纤维组织较少。这一发现表明PEMF刺激对骨折愈合诱导成骨和降低延迟愈合风险有作用。
