Bjerke Ingvild E, Yates Sharon C, Laja Arthur, Witter Menno P, Puchades Maja A, Bjaalie Jan G, Leergaard Trygve B
Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
Kavli Institute for Systems Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway.
iScience. 2020 Dec 8;24(1):101906. doi: 10.1016/j.isci.2020.101906. eCollection 2021 Jan 22.
The calcium-binding proteins parvalbumin and calbindin are expressed in neuronal populations regulating brain networks involved in spatial navigation, memory processes, and social interactions. Information about the numbers of these neurons across brain regions is required to understand their functional roles but is scarcely available. Employing semi-automated image analysis, we performed brain-wide analysis of immunohistochemically stained parvalbumin and calbindin sections and show that these neurons distribute in complementary patterns across the mouse brain. Parvalbumin neurons dominate in areas related to sensorimotor processing and navigation, whereas calbindin neurons prevail in regions reflecting behavioral states. We also find that parvalbumin neurons distribute according to similar principles in the hippocampal region of the rat and mouse brain. We validated our results against manual counts and evaluated variability of results among researchers. Comparison of our results to previous reports showed that neuron numbers vary, whereas patterns of relative densities and numbers are consistent.
钙结合蛋白小白蛋白和钙结合蛋白在调节参与空间导航、记忆过程和社会互动的脑网络的神经元群体中表达。要了解这些神经元的功能作用，需要有关它们在整个脑区中的数量信息，但目前几乎没有此类信息。我们采用半自动图像分析方法，对免疫组化染色的小白蛋白和钙结合蛋白切片进行全脑分析，结果表明这些神经元在小鼠脑中以互补模式分布。小白蛋白神经元在与感觉运动处理和导航相关的区域占主导，而钙结合蛋白神经元在反映行为状态的区域占优势。我们还发现，小白蛋白神经元在大鼠和小鼠脑的海马区按照相似的原则分布。我们对照人工计数验证了我们的结果，并评估了研究人员之间结果的可变性。将我们的结果与之前的报告进行比较表明，神经元数量存在差异，而相对密度和数量模式是一致的。
